1997 Fiscal Year Final Research Report Summary
Minimal residual leukemia in autologous peripheral blood stem cell transplantation
| Project/Area Number |
08670902
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | YOKOHAMA CITY UNIVERSITY |
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Principal Investigator |
IKUTA Koichiro Yokohama City Univ., School of Med., Assistant Professor, 医学部, 講師 (80159590)
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| Co-Investigator(Kenkyū-buntansha) |
FUNABIKI Tetsunori Yokohama City Univ., School of Med., Instructor, 医学部, 助手 (20264616)
SASAKI Hideki Yokohama City Univ., School of Med., Associate Professor, 医学部, 助教授 (50106316)
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| Project Period (FY) |
1996 – 1997
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| Keywords | acute leukemia / PBSCT / PBSC / MRD / IgH / TCR / RT-PCR / chimeric RNA |
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| Research Abstract |
We investigated minimal residual disease (MRD) in autologous peripheral blood stem cell transplantation (PBSCT) for acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML) in children. MRD can be defined as disease occurring at a subclinical level and beyond detection by conventional methods of assessment. Application of the polymerase chain reaction (PCR) to the hypervariable segment of the immunoglobulin heavy chain (IgH) gene or T-lymphocyte receptor gene and RT-PCR of specific chimera messenger RNA,allows detection of MRD in ALL and AML at a level of one leukemic cell in 10^4-10^5 normal marrow cells. The detection of MRD of harvested peripheral blood stem cell (PBSC) have a value in the indication of autologous PBSCT.We detected MRD in the harvested PBSC after complete remission for 3 cases of ALL and 4 cases of AML.In a case of AML with t(8 ; 21)(q22 ; q22), chimeric RNA was detected in PBSC.In other cases, MRD was not detected in PBSC.These 7 patients with acute leukemia were performed autologous PBSCT.Six patients have sustained complete remission without sign of MRD after transplantation. But in a case of AML with t(8 ; 21)(q22 ; q22) who has chimeric RNA in her PBSC,bone marrow relapse occurred 6 month after transplantation.
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[Publications] Xiao-Tang Kong, Kohmei Ida, Hitoshi Ichikawa, Kimiko Shimizu, Misao Ohki, Nobuo Maseki, Yasuhiko Kaneko, Masahiro Sako, Yukio Kobayashi, Arinobu Toujou, Ikuo Miura, Harumi Kakuda: "Tetsunori Funabiki, Keizo Horibe, Hiroyuki Hamaguchi, Yuichi Akiyama, Fumio Bessho, Masayoshi Yanagisawa, and Yasuhide Hayashi" Blood. 90. 1192-1199 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sasaki H,Matsuda M,Lu Y,Ikuta K,Matsuyama S,Hirabayashi Y,Mitsui H,Matsumura Y,Muramatsu M,Tsukada T,Aizawa S,Inoue T: "A fraction unresponsive to growth inhibition by TGF-beta among the high-proliferative potential progenitor cells in bone marrow of p53-deficient mice." Leukemia. 11. 239-244 (1997)
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[Publications] Fujisawa S,Maruta A,Sakai R,Taguchi J,Tomita N,Ogawa K,Kodama F,Takahashi K,Shibayama S,Kobayashi S,Ikuta K and Okubo T: "Pure red cell aplasia after major ABO-incompatible bone marrow transplantation : two case reports of treatment with recombinant human erythropoietin." Transplant Int. 9. 506-508 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Hideki MIYAMOTO,Miho OIDA,Hideyuki KIKUCHI,Keiji JOZAKI,Yuko YAMAKAWA,Naoko BABA,Tetsuo NAGATANI,Hiroshi NAKAJIMA,Koichiro IKUTA,Schichiro MIYAZAWA: "Diagnosis cingenital self-healing reticulohistiocytosis : report of a case with multiple, localized lesions." Eur J Dermatol. 6. 389-391 (1996)
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[Publications] Ohara A,Kojima S,Hamajima Y,Tsuchida M,Imashuku S.Ohta S,Sasaki H,okamura J,Sugita K,Kigasawa H,Kiriyama Y,Akatsuka J,Tsukimoto I: "Myelodysplastic syndrome and acute myelogenous leukemia as a late clonal complication in children with acquired aplastic anemia." Blood. 90. 1009-1013 (1997)
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[Publications] Sumita H,Ikuta K,Yokota S,Fujioka K,Takahashi H,Sekiguchi H,Hanzawa N,Kai S,Funabiki T,Okuyama T,Sasaki H,Matsuyama S: "Interferon therapy for hepatitis C virus infection with malignant disease in childhood." 100. 888-894 (1996)
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[Publications] Funabiki T,Sato A,Matsuda M,Watanabe Y,Goto A,Goto H,Sumita H,Sekiguchi H,Hanzawa N,Kai S,Ikuta K,Sasaki H,Matsuyama S: "Allogeneic bone marrow transplantation for advanced neuroblastoma patients with renal falure." J J Pediat Oncol. 33. 548-551 (1996)
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[Publications] Goto H,Goto A,Watanabe Y,Matsuda M,Hanzawa N,Sekiguchi H,Kai S,Funabiki T,Ikuta K,Sasaki H,Matsuyama S: "Allogeneic peripheral blood stem-cell transplantation for pediatric acute leukemia and malignant lymphoma" J J Pedat Hematol. 11. 356-362 (1997)
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