1998 Fiscal Year Annual Research Report

角化細胞活性化酸素産生に関わる膜情報伝達系Ca^<2+>, Cキナーゼ活性化機構の解析

Research Project

Project/Area Number	08670937
Research Institution	Hokkaido University
Principal Investigator	小泉洋子北海道大学, 医学部・附属病院, 講師 (60113552)
Keywords	角化細胞 / 活性酸素 / カルシウム / C-キナーゼ / H_2O_2
Research Abstract	細胞内では紫外線,放射線,薬剤等によりO2-が生じsuperoxide dismutaseによりH202に変換される.これまで表皮角化細胞のH202を直接測定し,UV照射が細胞内H202を容量依存性に増加させることを報告した.また,TPAによる角化細胞のH202産生が報告され,著者が研究してきたprotein kinase C活性化,細胞内遊離カルシウム濃度上昇刺激は,活性酸素産生を伴うと考えられる. 紫外線により表皮で産生され,免疫抑制作用を有するCis-urocanic acidは角化細胞ヒスタミン受容体の反応性を抑制することを報告したが,細胞内遊離カルシウム上昇を調節するヒト角化細胞ヒスタミン受容体をagonist,antagonistを用いて検討し,H2受容体が関わっていることを見い出した(J Dermatol Sci,in print). さらにサイトカインとその受容体の角化細胞での局在,遊離調節機構を検討した.角化細胞はstem cell factor(SCF)を生成遊離して肥満細胞やメラノサイトの増殖や機能を調節する・抗体で二重染色するとSCFの受容体Kitは角化細胞に発現しておらず,遊離されたSCFは続いての遊離を直接調節しないと考えられる.角化細胞からのSCFのmRNA,蛋白の生成はグルココルチコイド,ヒスタミンにより抑制される(J Invest Derinatol 1998).さらにIL-18について検討した.IL-18はinterferon-γ誘導因子である.乾癖にはinterferon-γを産生するThlリンパ球が炎症反応に携わっており,IL-18の角化細胞における解析は乾癬の研究に重要である.IL-18とILl8受容体のヒト培養角化細胞、皮膚における発現を免疫組織化学,immunoblot法により確認した(J Invest Dermatol in print).

Research Products
(16 results)

All Other

All Publications (16 results)

[Publications] Koizumi H: "Cis-urocanic acid attenuates histamine receptor-mediated activation of adenylate cyclase and increase of intracellular Ca^<2+>" Arch Dermatol Res. 290. 264-269 (1998)
[Publications] Shimizu T: "Cis-urocanic acid down-regulates histamine-mediated activation of adenylate cyclase in pig epdermis." Acta Derm Venereol(Stockh). 78. 348-350 (1998)
[Publications] Koizumi H: "Glucocorticoid decreases stem cell factor production in keratinocytes." J Invest Dermatol. 110. 628 (1998)
[Publications] Hagari Y: "Nodular localized cutaneous amyloidosis : further demonstration of monoclonality of infiltrating plasma cells in four additional Japanese patients." Br J Dermatol. 158. 652-654 (1998)
[Publications] Koizumi H: "CD34 positive dendritic cells are intrinsic part of smooth muscle hamartoma." Br J Dermatol. (in print).
[Publications] Koizumi H: "H2 histamine receptor-mediated increase in intracellular Ca^<2+> in cultured human keratinocytes." J Dermatol Sci. (in print).
[Publications] Koizumi H: "Adnexal polyp of neonatal skin observed beyond the neonatal period." Acta Derm Venereol(Stockh). 78. 391-392 (1998)
[Publications] Koizumi H: "Congenital onychodysplasia of the index finger with anomalt of the great toe." Acta Derm Venereol. 78. 478-479 (1998)
[Publications] Namba Y: "Specific cutaneous lesions of the scalp in myelodysplastic syndrome with deletion of 20q." J Dermatol. (in print).
[Publications] Sato-Natsumura KC: "Analysis of c-kit exon 11 and exon 17 of urticaria pigmentosa that occured in monozygotic twin sisters." Br J Dermatol. (in print).
[Publications] 小泉洋子: "増殖とadenosine deaminase.皮膚疾患と酵素up to date." Monthly Book Derma. (印刷中).
[Publications] Koizumi H: "IL-18 and IL-18 rceptor expression in human keratinocytes." J Invest Dematology. (in print).
[Publications] 小泉洋子: "皮膚病理をよむ" 文光堂, (1998)
[Publications] 小泉洋子: "皮膚疾患最新の治療'1999-2000" 南江堂, (1999)
[Publications] 小泉洋子: "Key Word 1999-2000 皮膚疾患" 先端医学社 (印刷中),
[Publications] 小泉洋子: "皮膚科専門医テキスト" 南江堂 (印刷中),

1998 Fiscal Year Annual Research Report

角化細胞活性化酸素産生に関わる膜情報伝達系Ca^<2+>, Cキナーゼ活性化機構の解析

Principal Investigator

小泉 洋子 北海道大学, 医学部・附属病院, 講師 (60113552)

Research Products

[Publications] Koizumi H: "Cis-urocanic acid attenuates histamine receptor-mediated activation of adenylate cyclase and increase of intracellular Ca^<2+>" Arch Dermatol Res. 290. 264-269 (1998)

[Publications] Shimizu T: "Cis-urocanic acid down-regulates histamine-mediated activation of adenylate cyclase in pig epdermis." Acta Derm Venereol(Stockh). 78. 348-350 (1998)

[Publications] Koizumi H: "Glucocorticoid decreases stem cell factor production in keratinocytes." J Invest Dermatol. 110. 628 (1998)

[Publications] Hagari Y: "Nodular localized cutaneous amyloidosis : further demonstration of monoclonality of infiltrating plasma cells in four additional Japanese patients." Br J Dermatol. 158. 652-654 (1998)

[Publications] Koizumi H: "CD34 positive dendritic cells are intrinsic part of smooth muscle hamartoma." Br J Dermatol. (in print).

[Publications] Koizumi H: "H2 histamine receptor-mediated increase in intracellular Ca^<2+> in cultured human keratinocytes." J Dermatol Sci. (in print).

[Publications] Koizumi H: "Adnexal polyp of neonatal skin observed beyond the neonatal period." Acta Derm Venereol(Stockh). 78. 391-392 (1998)

[Publications] Koizumi H: "Congenital onychodysplasia of the index finger with anomalt of the great toe." Acta Derm Venereol. 78. 478-479 (1998)

[Publications] Namba Y: "Specific cutaneous lesions of the scalp in myelodysplastic syndrome with deletion of 20q." J Dermatol. (in print).

[Publications] Sato-Natsumura KC: "Analysis of c-kit exon 11 and exon 17 of urticaria pigmentosa that occured in monozygotic twin sisters." Br J Dermatol. (in print).

[Publications] 小泉洋子: "増殖とadenosine deaminase.皮膚疾患と酵素up to date." Monthly Book Derma. (印刷中).

[Publications] Koizumi H: "IL-18 and IL-18 rceptor expression in human keratinocytes." J Invest Dematology. (in print).

[Publications] 小泉洋子: "皮膚病理をよむ" 文光堂, (1998)

[Publications] 小泉洋子: "皮膚疾患最新の治療'1999-2000" 南江堂, (1999)

[Publications] 小泉洋子: "Key Word 1999-2000 皮膚疾患" 先端医学社 (印刷中),

[Publications] 小泉洋子: "皮膚科専門医テキスト" 南江堂 (印刷中),

小泉洋子北海道大学, 医学部・附属病院, 講師 (60113552)