Studies on the model for cultured human epidermal allong rafting using hu-PBL-SCID mice

1997 Fiscal Year Final Research Report Summary

• Project/Area Number: 08670954
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Niigata University
• Principal Investigator: KAWAI Kazuhiro Niigata University School of Medicine, Department of Dermatology, Research Associate, 医学部, 助手 (90242411)
• Project Period (FY): 1996 – 1997
• Keywords: hu-PBL-SCID mice / cultured epidermis / skin allograft

Research Abstract

To determine whether Langerhans cell-free cultured human epidermal grafts survive for a prolonged period in allogeneic recipients, an experimental model using SCID mice reconstituted with human peripheral blood lymphocytes (hu-PBL) was established. In this model, hu-PBL were activated with anti-CD3 mAb in vitro and then transferred into SCID mice pretreated with anti-asialoGM1 Ab in vivo. Flow cytometry of the peripheral lymphoid organs demonstrated engraftment of hu-PBL (mainly T cells) 2-4 weeks after the transfer. Cultured human epidermal grafts were accepted by untreated by untreated SCID mice, when transplanted on the dorsal muscle and protected with a silicon transplantation chamber. Ten days after cultured human epidermal grafting on SCID mice reconstituted with allongeneic hu-PBL,however, detachment of the epidermis and infiltrantion of human CD3+ T cells in the graft bed were histologically confirmed. These results suggest that mice. This model will be usefull for analysis of the rejection mechanisms of cultured human epidermal allonrafts.