1997 Fiscal Year Final Research Report Summary
Establishment of animal models for atopic dermatitis and induction of oral tolerance
| Project/Area Number |
08670982
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kyorin University |
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Principal Investigator |
HAYAKAWA Kazuhito Kyorin University School of Medicine, Lecturer, 医学部, 講師 (50146669)
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| Project Period (FY) |
1996 – 1997
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| Keywords | atopic dermatitis / animal model / oral tolerance / cytokine / mRNA / RT-PCR method / in situ hybridization |
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| Research Abstract |
BALB/C mice were sensitized with 2,4,6-trinitro-chlorobenz ine, and then were repeatedly elicited on the original sensitized site with the same antigen at 2-day intervals for 24 days. To investigate the differences in the cutaneous cytokine milieu between the acute and chronic phases of contact hypersensitivity, sequential cytokine dynamics after 2,4,6-trinitro-chlorobenzine application were assessed in the acute vs chronic lesions, using RT-PCR technique. As a result, increased mRNA levels for Th1-type cytokines (IL-2, IFN-gamma) were observed in the acute lesions, whereas those for Th2-type cytokines (IL-4, IL-10) were markedly up-regulated in the chronic lesions.
Similar study with BALB/C mice sensitized by subcutaneous nickel injection also showed that repeated applications of nickel gave rise to a shift from Th1 to Th2 dominated responses. Oral administration of nickel prior to repeated elicitation suppressed development of dermatitis. We demonstrated Th2-type responses in the repeatedly elicited sites, using RT-PCR technique.
Subsequently, BALB/C mice were sensitized with 2,4,6-trinitro-chlorobenzine and then were repeatedly elicited on the original sensitized site with the same antigen at 2-day intervals for 24 days. Using in-situ hybridization technique, we examined time course and localization of expression for IFN-gamma mRNA and IL-10mRNA.In the acute lesions, we demonstrated increased expression for IFN-gamma mRNA on T cells in the dermis between several and 12 hours. Expression of IL-10mRNA on T cells in the dermis was up-regulated between 10 and 24 hours. In the chronic lesions, expression of IL-10mRNA was observed on infiltrating T cells into epidermis and dermis at 3-24 hours.
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