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1998 Fiscal Year Final Research Report Summary

Analysis of dicbetic angiopetry-study on expression of VEGF

Research Project

Project/Area Number 08671133
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionUniversity of Tsukuba

Principal Investigator

OKUDA Yukichi  Associate Professor Institute of Clinical Medicine University of Tsukuba, 臨床医学系, 助教授 (60211132)

Project Period (FY) 1996 – 1998
KeywordsDiabetes / VEGF / Diabetic retinopethy / Hypoxia / Cultured endothelial and smooth muscle cells / Sports medicine
Research Abstract

We determined the levels of vascular xxxxxx (grooooowth factor/vascular permeability factor (VFGF/VPF) in the aqueous and serum in non-insulin dependent dixxx patients with proliterative retinopathy and neovascular glancoma, and normal subjects. The levels of VEGF were very high in patients with diabetic patients with retinopathy and subjects with physical training at high altitudes.
Also, hypoxia and endothelin-I constitute potent stimuli for VEGF production in cultured vascular cells.

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Y.Okuda et al.: "Hypoxia and Endothelin-I induce VEGF production in Human Vascular smooth muscle cells" Life Sciences. 63. 477-484 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Asano,Y.Okuda et al.: "Increase in Seron Vascular endothelial growth factor levels during altitude training" Acta Physiol Scand. 162. 477-484 (1998)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Hazama,Y.Okuda et al.: "W-3 poly unsaturated tatty acids-modulation of voltape-dependent L-type Ca^<2+> current in guinea-pig trudesl smooth muscle cells" European Journal of Pharmacology. 355. 257-266 (1998)

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      「研究成果報告書概要(和文)」より
  • [Publications] M.Asano,Y.Okuda et al.: "Influence of Cellular incorporction of n-3 eicuscpetae-muic acid on moracelluler Ca^<2+> concentrotwn and monbrne potent in vascular smooth muscle cells" Atherosclerosis. 138. 117-127 (1998)

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      「研究成果報告書概要(和文)」より
  • [Publications] Y.Asakura,Y.Okuda et al.: "Pestenosis after percutaneous transluminal coronary congioplasty in potients with non insulin dependent diadetes mellitous(NIDDM)" Journal of Cardiovaecular Risk. 5. 331-334 (1998)

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      「研究成果報告書概要(和文)」より
  • [Publications] Y.Tachi,Y.Okuda et al.: "High Concentration of glucose cause impairment of the function of the pluthehione redox cycle in human vascular smooth muscle cells" FFBS Letiers. 421. 19-22 (1998)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Sone,Y.Okuda et al.: "Vascular Endothelil Growth Factor levl in Aqueous Humor of Diabetic patients with Rubeotic Glaucoma is marketing Eleliter" Diabetes Care. Vol.19 No.11. 1306-1307 (1996)

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  • [Publications] H.Sone,Y.Okuda et al.: "Progesterone induces vascular endothelial growth facter on retinal pigment epithelial cells in culture" Life Science. Vol.59 No.1. 21-25 (1996)

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      「研究成果報告書概要(和文)」より
  • [Publications] H.Sone,Y.Kuwakami,Y.Okuda et al.: "Vascular endothelial growth factor is induced by long-term high glucose and up-regulated by Acute glucose depriration in cult povine Rotinal Pigmonted Epithelil cells" Biochemical and Biophysical Research Communiccbn. Vol.221. 193-198 (1996)

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  • [Publications] 小沢忠彦、奥田諭吉 他: "糖尿病性網膜症と糖尿病性血管新生緑内障における利尿水と血中の血〓内〓〓〓因子/血〓〓〓性因子〓〓" 日本眼科学会雑誌, 731-738 (1998)

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  • [Publications] Hiroshi Sone, Yukichi Okuda, Yasushi Kawakami, Shinichi Kondo, Mitsuya Hanatani, Katsuhiko Matsuo, Hideo Suzuki, Kamejiro Yamashita: "Progesterone induces vascular endothelial growth factor on retinal pigment epithelial cells in culture" Life Sciences. 59 (1). 21-25 (1996)

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  • [Publications] Hirohito Sone, Yasushi Kawakami, Yukichi Okuda, Shinichi Kondo, Mitsuya Hanatani, Hideo Suzuki, Kamejiro Yamashita: "Vascular endothelial growth factor is induced by long-term high glucose concentration and up-regulated by acute glucose deprivation in cultured bovine retinal pigmented epithelial cells" Biochemical and Biophysical Research Communications. 221. 193-198 (1996)

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  • [Publications] Yukichi Okuda, Horacio J.Adrogue, Toshiaki Nakajima, Masakazu Mizutani, Michiko Asano, Yoichi Tachi, Seiji Suzuki, Kamejiro Yamashita: "Increased production of PDGF by angiotensin and high glucose in human vascular endothelium" Life Sciences. 59(17). 1455-1461 (1996)

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  • [Publications] Yukichi Okuda, Masakazu Mizutani, Masashi Ogawa, Hirohito Sone, Michiko Asano, Yukari Asakura, Masaaki Isaka, Seiji Suzuki, Yasushi Kawakami, James B.Field, Kamejiro Yamashita: "Long-term efects of ecosapentaenoic acid on diabetic peripheral neuropathy and serum lipids in patients with Type II diabetes mellitus" Journal of Diabetes and Its Complications. 9. 280-287 (1996)

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  • [Publications] Masakazu Mizutani, Yukichi Okuda Kamejiro Yamashita: "Effect of cilostazol on the production of platelet-derived growth factor in cultured human vascular endothelial cells" Biochemical and Molecular Medicine. 57. 156-158 (1996)

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  • [Publications] Hirohito Sone, Yukichi Okuda, Yasushi Kawakami, Kamejiro Yamashita: "Effects of high glucose concentration and a thromboxane synthase inhibitor on the production of thromboxane A_2 and prostaglandin I_2 and E_2 by cultured retinal endothelial cells" Life Science. 58 (3). 239-243 (1996)

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  • [Publications] Hirohito Sone, Yukichi Okuda, Yasushi Kawakami, Mitsuya Hanatani, Hideo Suzuki, Tadahiko Kozawa, Sachiko Honmura, Kamejiro Yamashita: "Vascular endothelial growth factor level In aqueous humor of diabetic patients with rubeotic glaucoma is markedly elevated" Diabetes care. 19 (11). 1306-1307 (1996)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Hirohito Sone, Yukichi Okuda, Takashi Yamaoka, Yasushi Kawakami, Masato Odawara, Teruhiko Mtsushima, Koichi Kawai, Kamejiro Yamashita: "Pedicting long-term glycemic control of post-educational type II diabetic patients by evaluating serum 1,5-anhydroglucitol levels" Diabetes Research and Clinical Practice. 34. 83-88 (1996)

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  • [Publications] Yukichi Okuda, kazuko Kawashima, Seiji Suzuki, Yukari Asakura, Michiko Asano, Kazuki Tsurumaru, Haiqing Dai, Yoichi Tachi, Chieko Bannai, Masahiro Saitoh, Kamejiro Yamashita: "Restoration of nitric oxide production by aldose reductase inhibitor in human endothelial cell cultured in high-glucose medium" Life Science. 60 (3). 53-56 (1997)

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  • [Publications] Yukichi Okuda, Kazuko Kawashima, Toshie Sawada, Kazuki Tsurumaru, Michiko Asano, Seiji Suzuki, Masaaki Sonma, Toshiaki Nakajima, Kamejiro Yamashita: "Eicosapentaenoic and enhances nitric oxide production by cultured human endothelial cells" Biochemical and Biophysical Research Communications. 232 (2). 487-4491 (1997)

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  • [Publications] Hirohito Sone, Yasushi Kawakami, Y.Sekine, S.Honmura, K.Matsuo, T.Segawa, Hideo Suzuki, Yukichi Okuda, Kamejiro Yamashita: "Ocular vascular endothelial growth factor levels in diabetic rats are elevated before observable retinal proliferative chages" Diabetologia. 40. 726-730 (1997)

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  • [Publications] Toshiaki Nakajima, Kuniaki Iwasawa, Hisanori Hazama, Michiko Asano, Yukichi Okuda, Masao Omata: "Extracellular Mg^<2-> inhibits receptor-mediated Ca^<2->-permeable non-selective.Cation currents in aortic smooth mucle cells" European Journal of Pharmacology. 320. 81-86 (1997)

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  • [Publications] Michiko Asano, Toshiaki Nakajima, Kuniaki Iwasawa, Hisanori Hazama, Masao Omata, Masaaki Soma, Kamejiro Yamashita, Yukichi Okuda: "Inhibitory effects of omega-3 polyunsaturated fatty acids on receptor-mediated non-selective cation channels in rat A7r5 vascular smooth muscle cells" British Journal of Pharmacology. 120. 1367-1375 (1997)

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  • [Publications] Masakazu Mizutani, Michiko Asano, Sayon Roy, Toshiaki Nakajima, Masaaki Soma, Kamejiro Yamashita, Yukichi Okuda: "omega-3 Polyunsaturated fatty acids inhibit migration of human vascular smooth muscle cells in vitro" Life Sciences. 61 (19). 269-274 (1997)

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  • [Publications] Hirohito Sone, Yukichi Okuda, Yoko Tsurushima, Michiko Asano, Seiji Suzuki, Yasushi Kawakami, Kamejiro Yamashita: "Acute effects of thromboxane dual blocker (KDI-792) on different portions of lower limb blood flow-A study using doppler ultrasonography and laser doppler flowmetry in type 2 diabetic patients" Prostaglandins. 53. 395-409 (1997)

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  • [Publications] Hirohito Sone, Yasushi Kawakami, M.Hanatani, K.Matsuo, H.Suzuki, Yukichi Okuda, Kamejiro Yamashita: "Progesterone induces vascular endothelial growth factor on retinal pigment epithelial cells in culture Diabetes mellitus : New aspects of pathogenesis and treatment." Eds : T.Kaneko, K.B.Huh, M.Shichiri, Y.Oka. (]SY.encircledC.[)Nishimura/Smith-Gordon. 194-197 (1997)

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  • [Publications] Y.Asakura, Y.Okuda, T.Nakajima, M.Asano, S.Suzuki, K.Yamashita: "Effects of Benidipine Hydrochloride on DNA Synthesis and Cytosolic Free Ca^<2-> Concentrations of Rat Aortic Smooth Muscle Cells Induced by Insulin" Diabetes Research. 32. 237-244 (1997)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Yoichi Tachi, Yukichi Okuda, Chieko Bannai, Naomichi Okamura, Shiro Bannai, Kamejiro Yamashita: "High concentration of glucose causes impairment of the function of the glutathione redox cycle in human vascular smooth muscle cells" Febs Letters. 421 (1). 19-22 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Asano, K.Kaneoka, T.Nomura, K.Asano, H.Sone, K.Tsurumaru, K.Yamashita, K.Matsuno, H.Suzuki, and Y.Okuda: "Increase in serum vascular endothelial growth factor levles during altitude training" Acta Physiol Scand. 162. 455-459 (1998)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Michiko Asano, Toshiaki Nakajima, Hisanori Hazama, Kunisaki Iwasawa, Takanobu Tomaru, Masao Omata, Masaaki Soma, Yukari Asakura, Masakazu Mizutani, Seiji Suzuki, Kamejiro Yamashita, Yukichi Okuda: "Influence of cellular incorporation of n-3 eicosapetaenoic acid on intracellular Ca^<2-> concentration and membrane potential in vascular smooth muscle cells" Atherosclerosis. 138. 117-127 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisanori Hazama, Toshiaki Nakajima, Michiko Asano, Kuniaki Iwasawa, Toshihiro Morita, Kouichi Igarashi, Taiji Nagata, Tadashi Horiuchi, Junichi Suzuki, Masaaki Soma, Yukichi Okuda: "omega-3 polyunsaturated fatty acids-modulation of voltage-dependent 1.-type Ca^<2-> current in guinea-pig tracheal smooth muscle cells" European Journal of Pharmacology. 355. 257-266 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yukichi Okuda, Kazuki Tsurumaru, Seiji Suzuki, Takashi Miyauchi, Michiko Asano, Ying Hong, Hirohito Sone, Rie Fujita, Masakazu Mizutani, Yasushi Kawakami, Toshiaki Nakajima, Masaaki Soma, Katsuhiko Matsuo, Hideo Suzuki, Kamejiro Yamashita: "Hypoxia and endothelin-1 induce VEGF production in human vascular smooth muscle cells" Life Sciences. 63 (6). 477-484 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yukari Asakura, Masaaki Suzuki, Hiroshi Nonogi, Kazuo Haze, Akira Sato, Hiroshi Inada, Yukichi Okuda, Kamejiro Yamashita and Yutaka Harano: "Restenosis after percutaneous transluminal coronary angioplasty in patients with non-insulin-dependent diabetes mellitus (NIDDM)" Journal of Cardiovascular Risk. 5. 331-334 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigeru Yatoh, Yasushi Kawakami, Masayuki Imai, Tadahiko Kozawa, Toshiaki Segawa, Hideo Suzuki, Kamejiro Yamashita, and Yukichi Okuda: "Effect of a topically applied neutralizing antibody against vascular endothellal growth factor on corneal allograft rejection of rat" Transplantation. 66 (11). 1519-1524 (1998)

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Published: 1999-12-08  

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