1997 Fiscal Year Final Research Report Summary
The analysis of molecular mechanisms of autoimmune thyroid diseases
| Project/Area Number |
08671166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内分泌・代謝学
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| Research Institution | Kyushu University |
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Principal Investigator |
KOMAKI Gen Department of Psychosomatic Medicine, Kyushu University, Assistant Professor, 医学部, 助手 (70225564)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIKAWAJI Nobuhiro Medical Institute of Bioregulation, Kyushu University, Associate Professor, 生体防御医学研究所, 助教授 (90224659)
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| Project Period (FY) |
1996 – 1997
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| Keywords | HLA / Graves' disease / HLA-A2 / CTL / peptide |
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| Research Abstract |
The identification of T cell antigen epitopes is critical to reveal the etiology of autoimmune thyroid diseases. We have previously reported that Graves' disease are associated with HLA-A^<**>0206 and Hashimoto's thyroiditis are associated with HLA-A^<**>0207. We have also revealed that the allele-specific peptide-motifs including the dominant anchor amino acid residues were considerably different among these HLA-A2 subtypes. Based on the HLA-A^<**>0206 binding motif we synthesized peptides of TSHR which fit the motif and examined the T cell response against these peptides. However, we could not find any responses. Peptide library methods, which include all possible peptide sequences offer a potentially powerful tool for the detection of antigenic peptides recognized by T cells. We next synthesized the peptide mixtures including 20^9 peptide species and evaluated the effectiveness of the library method to investigate the specificity of cytotoxic T cells (CTL). We found that the mixture peptides are able to expand T cells from healthy donors and have peptides specific cytotoxicity suggesting that the library methods presents a powerful tool for monitoring specificity of T cells in autoimmune thyroid grands.
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