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1997 Fiscal Year Final Research Report Summary

Research mechanisms of Thyrotropin Receptor Expression

Research Project

Project/Area Number 08671169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionKyushu University

Principal Investigator

IKUYAMA Shoichiro  Kyushu University Faculty of Medicine, Assistant Prof., 医学部, 助手 (20184393)

Project Period (FY) 1996 – 1997
KeywordsTSH receptor / MHC class II / Y box protein / nicotinamide
Research Abstract

In a previous project, we have characterized structure and function of the promoter region of the rat thyrotropin (TSH) receptor gene, and identified several cis-acting elements and trans-acting factors which regulate the expression of the gene in thyroid. One of notable findings is that a transcription factor TSEP-1/YB-1, which acts on major histocompatibility complex class II gene as a repressor, also functions as a repressor on the TSH receptor promoter. Based on these studies, we further analyzed the promoter region and factors interacting with it in the present project.
We further cloned and characterized -4.2 kb region of the rat TSH receptor promoter. The -4.2 kb region exhibited less promoter activity than that of the minimal promoter region which we already analyzed, supporting the importance of the minimal promoter region on the activity. There are many putative TSEP-1/YB-1 binding sites, which function as a repressor, in the upstream region. These elements may suppress the promoter activity constitutively.
Aberrant expression of MHC class II antigen on thyroid cells is important for induction and perpetuation of autoimmune reaction in autoimmune thyroid diseases (AITD). TSH receptor itself is also important as an autoantigen. To control the expression of TSH receptor and MHC class II on thyroid cells, we focused on the action of TSEP-1/YB-1 on both genes. We showed that nicotinamide potentiated promoter activity of both genes, and this was attributed a dose-dependent reduction of TSEP-1/YB-1 expression induced by nicotinamide. This result does not directly implicate a therapeutic value of nicotinamide, but suggests the presence of agents which may regulate the expression of both genes simultaneously. If there were such agent which could repress the expression of TSH receptor and MHC class II on thyroid cells, it could be beneficial for treatment of AITD such aS Graves' disease. We are pursuing this possibility in the future project.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Ikuyama S, et sl: "Molecular biology of thyrotropin(TSH)receptor" Proceedings of the 2nd Fukuoka International Symposium on Medical Science.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikuyama S, et sl: "Cloning and characterization of the 4.2kb region of the rat thyrotropin receptor promoter" Endocrine J.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohe K, et al: "Interferon-γ suppresses thyrotropin receptor promoter activity by reducing thyroid transcription factor-1(TTF-1)binding to its recognition site" Mol Endocrinol.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikuyama S,et al.: "Molecular biology of thyrotropin (TSH) receptor" Proceedings of 2nd Fukuoka International Symposium on Medical Science. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikuyama S,et al.: "Cloning and characterization of the 4.2 kb region of the rat thyrotropin receptor promoter" Endocrine J. 44. 247-256 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohe K,et al.: "Interferon-gamma suppresses thyrotropin receptor promoter activity by reducing thyroid transcription factor-1 (TTF-1) binding to its recognition site" Mol Endocrinol. 10. 826-836 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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