1998 Fiscal Year Annual Research Report

アポトーシス阻止に作用する遺伝子FAP-1の遺伝子

Research Project

Project/Area Number	08671250
Research Institution	Jichi Medical School
Principal Investigator	畠清彦自治医科大学, 医学部, 助教授 (80192699)
Co-Investigator(Kenkyū-buntansha)	富塚浩自治医科大学, 医学部, 講師 (00285785) 照井康仁自治医科大学, 医学部, 講師 (10285786) 大月哲也自治医科大学, 医学部, 講師 (60275691)
Keywords	アポトーシス / FAP-1 / T細胞性白血病 / Fasリガンド
Research Abstract	FAP-1(Fas-Associated Protein-1)は細胞死に関係する遺伝子のひとつであるが、血液幹細胞の1分画であるCD34陽性細胞でさらに分化マーカーによって4つの細分画に分けて細胞を集め、遺伝子の発現を検討した。FAP-1は顆粒球/マクロファージコロニー、赤芽球コロニーでは発現に差違を認めなかった。FAP-1はT細胞のアポトーシスに関与しているので、T細胞性白血病のアポトーシスの機序をさらに検討した。3つの蛋白が精製でき、1つは内皮細胞由来インターロイキン8(IL-8Eと略す)であった。内皮細胞とT細胞性白血病との相互作用を観察したところ、IL-8Eが放出され、アポトーシスが誘導された。この作用機序を検討中である。Fasリガンドによるアポトーシスは、細胞が活性化されないと誘発されないことから、その作用機序としてFAP-1よりもFasリガンドに対するdecoy receptorの発現増強が関与している報告が出ており、検討が重要と考えられた。

Research Products

(11 results)

All Other

All Publications (11 results)

[Publications] Nakamura M,Hatake K,et al.: "Single glycosyltransferase,core 2 b1-6-N-acetylglucosamiyltransferase,regulates cell surface sialyl-Lex expression level in human pre-B lymphocytic leukemia cell line KM3 treated with phorbolester." J Biol Chem. 273. 26779-26789 (1998)
[Publications] Suzu S,Hatake K,et al.: "Identification of alternatively spliced transcripts encoding murine macrophage colony-stimulating factor." Biochem Biophys Res Comm. 245. 120-126 (1998)
[Publications] Yamashita Y,Hatake K,et al: "Tec and JAK2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription." Blood. 91. 1496-1507 (1998)
[Publications] Terui Y,Hatake K.: "Activated endothelial cells induces apoptosis in leukemic cells by endothelial interleukin-8." Blood. 92. 2672-2680 (1998)
[Publications] Terui Y,Hatake K.: "Identification of a novel apoptosis-inducing factor derived from leukemic cells: Endothelial interleukin-8,but not monocyte-derived,induces apoptosis in leukemic cells." Biochem Biophy Res Comm. 243. 407-411 (1998)
[Publications] Terui Y,Hatake K,et al.: "Bcl-X is a Regulatory Factor of Apoptosis and Differentiation in Megakaryocytic Lineage Cells." Exp Hematol. 26. 236-244 (1998)
[Publications] Tomizuka H,Hatake K,et al.: "CD95 predicts responsiveness to tretinoin in acute promyelocytic leukemia." Int J Mol Med. 1. 207-211 (1998)
[Publications] Ikeda T,Hatake K,et al.: "IL-13 as well as IL-4 induces monocytes/macrophages and a monoblastic cell line(UG3) to differentiate into multinucleated giant cells in the presence of M-CSF." Biochem Biophys Res Comm. (in press). (1999)
[Publications] Igarashi H,Hatake K,Tomizuka H,et al.: "High serum level of macrophage colony-stumlating factor and granulocytic colony-stimulating factor in Kawasaki disease." Brit J Haematol. (in press). (1999)
[Publications] Tanaka-Douzono M,Hatake K,et al.: "The in vivo stimulatory effet of macrophage colony-stimulating factor on th number of stroma-initiating cells." J Cell Physiol. (in press). (1999)
[Publications] Shimura M,Hatake K,et al.: "Micronuclei formation and aneupioldy induced by Vpr,an accessory gene of human immunodeficiency virus type 1." FASEB J. (in press). (1999)

1998 Fiscal Year Annual Research Report

アポトーシス阻止に作用する遺伝子FAP-1の遺伝子

Principal Investigator

畠 清彦 自治医科大学, 医学部, 助教授 (80192699)

Research Products

[Publications] Nakamura M,Hatake K,et al.: "Single glycosyltransferase,core 2 b1-6-N-acetylglucosamiyltransferase,regulates cell surface sialyl-Lex expression level in human pre-B lymphocytic leukemia cell line KM3 treated with phorbolester." J Biol Chem. 273. 26779-26789 (1998)

[Publications] Suzu S,Hatake K,et al.: "Identification of alternatively spliced transcripts encoding murine macrophage colony-stimulating factor." Biochem Biophys Res Comm. 245. 120-126 (1998)

[Publications] Yamashita Y,Hatake K,et al: "Tec and JAK2 kinases cooperate to mediate cytokine-driven activation of c-fos transcription." Blood. 91. 1496-1507 (1998)

[Publications] Terui Y,Hatake K.: "Activated endothelial cells induces apoptosis in leukemic cells by endothelial interleukin-8." Blood. 92. 2672-2680 (1998)

[Publications] Terui Y,Hatake K.: "Identification of a novel apoptosis-inducing factor derived from leukemic cells: Endothelial interleukin-8,but not monocyte-derived,induces apoptosis in leukemic cells." Biochem Biophy Res Comm. 243. 407-411 (1998)

[Publications] Terui Y,Hatake K,et al.: "Bcl-X is a Regulatory Factor of Apoptosis and Differentiation in Megakaryocytic Lineage Cells." Exp Hematol. 26. 236-244 (1998)

[Publications] Tomizuka H,Hatake K,et al.: "CD95 predicts responsiveness to tretinoin in acute promyelocytic leukemia." Int J Mol Med. 1. 207-211 (1998)

[Publications] Ikeda T,Hatake K,et al.: "IL-13 as well as IL-4 induces monocytes/macrophages and a monoblastic cell line(UG3) to differentiate into multinucleated giant cells in the presence of M-CSF." Biochem Biophys Res Comm. (in press). (1999)

[Publications] Igarashi H,Hatake K,Tomizuka H,et al.: "High serum level of macrophage colony-stumlating factor and granulocytic colony-stimulating factor in Kawasaki disease." Brit J Haematol. (in press). (1999)

[Publications] Tanaka-Douzono M,Hatake K,et al.: "The in vivo stimulatory effet of macrophage colony-stimulating factor on th number of stroma-initiating cells." J Cell Physiol. (in press). (1999)

[Publications] Shimura M,Hatake K,et al.: "Micronuclei formation and aneupioldy induced by Vpr,an accessory gene of human immunodeficiency virus type 1." FASEB J. (in press). (1999)

畠清彦自治医科大学, 医学部, 助教授 (80192699)