1997 Fiscal Year Final Research Report Summary
Search for New Growth Associated Molecules Expressed on Cell Surface of Hematopoietic Stem Cells
| Project/Area Number |
08671255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Saitama Medical School |
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Principal Investigator |
MORI Shigehisa Saitama Medical School, Assistant Professor, 医学部, 講師 (10190993)
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| Project Period (FY) |
1996 – 1997
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| Keywords | hematopoietic cells / cell surface molecules / CD34 / homotypic aggregation |
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| Research Abstract |
The aim of this research project is to identify new genes encoding the proteins important for cell growth, among the cell surface molecules associated with homotypic aggregation.
We isolated mRNA from CD34 positive cell lines (KG-1 etc) and CD34 positive hematopoietic stem cells of the patients with hematological malignancy, and generated cDNA using oligo dT primer and randam primer. Several cDNA librarys were constructed from the cDNA and expression vector. These cDNA librarys were tranfered into the COS-7 cells by electroporation, and the cells that reacted with CD34 positive cells, were concentrated by the modified panning method. Two cDNA fragments were isolated from cDNA library of KG-1 cell using this system. The sequence analysis revieled that one of the cDNA fragments is identical to the gene of CD54 (ICAM-1 : intercellular adhesioin melecule 1).
The other cDNA fragment will analysed succesively. We will try to isolate new gene associated with homotypic aggregation using different cDNA librarys.
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[Publications] Muto, A., Mori, S., Matsushita, H., Awaya, N., Ueno, H., Takayama, N., Okamoto, S., Kizaki, M., and Ikeda, Y.: "Serial quantification of minimal residual disease of t(8 ; 21) acute myelogenous leukaemia with RT-competitive PCR assay" Br.J.Haematol. 95. 85-94 (1996)
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[Publications] Koide, J., Takada, K., Sugiura, M., Sekine, H., Ito, T., Saito, K., Mori, S., Takeuchi, T., Uchida, S., and Abe, T.: "Spontaneous establishment of an Epstein-Barr virus-infected fibroblast line from the synovial tissue of a rheumatoid arthritis patient" J.Virol.71. 2478-2481 (1997)
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[Publications] Kobayashi, H., Takemura, Y., Miyachi, H., Kawabata, M., Mori, S., Kawai, Y., Furihata, K., Sekiguchi, S., and Watanabe, K.: "Quantitative analysis of human multidrug resistance 1 (MDR1) gene expression by nonisotonic competitive reverse transcriptase polymerase chain reaction assay" J.Clin.Lab.Anal.11. 258-266 (1997)
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[Publications] Koide, J., Okusawa, E., Ito, T., Mori, S., Takeuchi, T., and Abe, T.: "Granulomatous amoebic encephalit is caused by acanthamoeba in a patient with systemic lupus erythematosus" Clin.Rheumatol.17. 329-332 (1998)
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[Publications] Pang, M., Abe, T., Fujihara, T., Mori, S., Tsuzaka, K., Amano, K., Koide, J., and Takeuchi, T.: "Up-regulation of aEb7, a novelintegrin adhesion molecule, on T cells from systemic lupus erythematosus patients with specific epithelial involvement" Arthritis Rheum. 41. 1456-1463 (1998)
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[Publications] Mori, S., Maruyama, H., Ito, I., Tokuhira, M., Koide, J., Takeuchi, T., Itoyama, S., Masunaga, A., Fukushima, M., Suzuki H., and Abe, T.: "Diagnosis of measles viral pneumonia in a patient with Hodgkin's disease by revese transcription-polymerase chain reaction of serum" Int.J.Hematol.68. 327-331 (1998)
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