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1997 Fiscal Year Final Research Report Summary

THE EXPRESSION AND ENHANCEMENT OF CO-STIMULATORY MOLECULE (B7-1, B7-2) IN GASTRO-INTESTINAL CANCER.

Research Project

Project/Area Number 08671377
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

HARAGUCHI Masaru  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY LECTURE, 生体防御医学研究所, 講師 (40228531)

Co-Investigator(Kenkyū-buntansha) HATAKENAKA Masamitsu  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY ASSISTANT, 生体防御医学研究所, 助手 (40253413)
YAMAGATA Motoyuki  MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY ASSITANT, 生体防御医学研究所, 助手 (90294975)
Project Period (FY) 1996 – 1997
KeywordsGASTROINTESTINAL CANCER / TUMOR IMMUNOLOGY / CO-STIMULATORY MOLECULE / CYTOKINE
Research Abstract

The co-stimulatory molecule (B7-1, B7-2) has been shown to play an important role in the induction of T cell mediated immunity against tumors. In the present study, we examined the expression of B7-1 and B7-2 in the human esophageal, gastric and colon cancer cell lines for mRNA level by a reverse transcriptase/polymerase chain reaction analysis (RT-PCR), for their surface expression by a flow-cytometric analysis with monoclonal antibodies. The expression of mRNA for B7-1 or B7-2 was detected in all 18 cell lines tested, except for B7-2 on one cell line. The cells from 13 (72%) or 12 (67%) of these cell lines expressed the surface B7-1 or B7-2 antigen, detected with respective mAb. The surface expression of B7-1 or B7-2 was increased in four to five of the six cell lines tested after a culture with interferon-gamma (IFN-g). In addition, the up-regulation of B7-1 or B7-2 expression by IFN-g was inhibited by interleukin-10 (IL-10). These results indicated that, in the cell lines derived from gastrointestinal carcinoma, both the co-stimulatory molecules B7-1 and B7-2 were detectable in the majority of the cell lines examined at the mRNA and protein levels, and could be regulated with cytokine IFN-g and IL-10.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Haraguchi, M.: "Clinical significance of pyrimidine nucleoside phosphorylase in colorectal carcinoma." Int J Oncol. 10. 493-496 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haraguchi, M.: "Clinical significance of tissue inhibitor of metalloproteinase expression in gastric carcinoma." Br J Cancer. 76. 531-536 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haraguchi, M.: "Analysis of ornithine decarboxylase mRNA expression in colorectal carcinoma." Dis Colon Rectum. 40. 1095-1100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haraguchi, M.: "Clinical significance of integrin alpha 6 mRNA expression in gastric carcinoma." Int J Oncol. 11. 959-964 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Haraguchi, M.: "Expression of MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mimori M., Mori M., Ueo H., Koba F., Nakashima H., Baba K., Haraguchi M., Akiyoshi T.: "Clinical significance of pyrimidine nucleoside phosphorylase in colorectal carcinoma." Int J Oncol. 10. 493-496 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimori K., Mori M., Shiraishi T., Baba K., Haraguchi M., Abe R., Ueo H., Akiyoshi T.: "Clinical significance of tissue inhibitor of metalloproteinase expression in gastric carcinoma." Br J Cancer. 76. 531-536 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimori K., Mori M., Shiraishi T., Fujie T., Baba K., Kusumoto H., Haraguchi M., Ueo H., Akiyoshi T.: "Analysis of ornithine decarboxylase mRNA expression in colorectal carcinoma." Dis Colo Rect. 40. 1095-1100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimori K.Mori M., Begum N.A., Tanaka S., Haraguchi M., Ueo H., Karimine N., Akiyoshi T., Barnard G.F.: "Clinical significance of integrin alpha 6 mRNA expression in gastric carinoma." Int J Oncol. 11. 959-964 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Li J., Yang Y., Fujie T., Tanaka F., Mimori K., Haraguchi M., Ueo H., Mori M., Akiyoshi T.: "Expression of MAGE gene family in human gastric carcinoma." Anticancer Res. 17. 3559-3564 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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