1997 Fiscal Year Final Research Report Summary
THE EXPRESSION AND ENHANCEMENT OF CO-STIMULATORY MOLECULE (B7-1, B7-2) IN GASTRO-INTESTINAL CANCER.
| Project/Area Number |
08671377
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
HARAGUCHI Masaru MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY LECTURE, 生体防御医学研究所, 講師 (40228531)
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| Co-Investigator(Kenkyū-buntansha) |
HATAKENAKA Masamitsu MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY ASSISTANT, 生体防御医学研究所, 助手 (40253413)
YAMAGATA Motoyuki MEDICAL INSTITUTE OF BIOREGULATION,KYUSHU UNIVERSITY ASSITANT, 生体防御医学研究所, 助手 (90294975)
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| Project Period (FY) |
1996 – 1997
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| Keywords | GASTROINTESTINAL CANCER / TUMOR IMMUNOLOGY / CO-STIMULATORY MOLECULE / CYTOKINE |
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| Research Abstract |
The co-stimulatory molecule (B7-1, B7-2) has been shown to play an important role in the induction of T cell mediated immunity against tumors. In the present study, we examined the expression of B7-1 and B7-2 in the human esophageal, gastric and colon cancer cell lines for mRNA level by a reverse transcriptase/polymerase chain reaction analysis (RT-PCR), for their surface expression by a flow-cytometric analysis with monoclonal antibodies. The expression of mRNA for B7-1 or B7-2 was detected in all 18 cell lines tested, except for B7-2 on one cell line. The cells from 13 (72%) or 12 (67%) of these cell lines expressed the surface B7-1 or B7-2 antigen, detected with respective mAb. The surface expression of B7-1 or B7-2 was increased in four to five of the six cell lines tested after a culture with interferon-gamma (IFN-g). In addition, the up-regulation of B7-1 or B7-2 expression by IFN-g was inhibited by interleukin-10 (IL-10). These results indicated that, in the cell lines derived from gastrointestinal carcinoma, both the co-stimulatory molecules B7-1 and B7-2 were detectable in the majority of the cell lines examined at the mRNA and protein levels, and could be regulated with cytokine IFN-g and IL-10.
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