1997 Fiscal Year Final Research Report Summary
A fundamental study of the bioartificial pancreas using polysulfone hollow fibers
| Project/Area Number |
08671379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
NAKAI Ichiro Kyoto Prefectural University of Medicine, Second Department of Surgery, Assistant Professor, 医学部, 助手 (30188867)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Islet transplantation / Xenotransplantation / Bioartificial Pancreas / Immunoisolation |
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| Research Abstract |
Following confirmation of biocompatibility of the fibers and good viability of isolated rat and hamster islets, morphological and functional change of the diffusion chamber type bioartificial pancreas made of polysulfone hollow fiber were investigated in vivo. The device was made of a bundle of polysulfone fibers (number of fibers : 180, inner diameter : 300mum, wall thickness : 80mum, pore size : 0.1mum, length 40 or 100mm) with seeding ports on both ends. Isolated syngeneic (Lewis rat) or xenogeneic (Syrian hamster) islets mixed up with RPMI medium and 2 % agarose were seeded into the devise, which was transplanted in the peritoneal cavity of streptozotocin-induced diabetic Lewis rats without immunosuppression. Fasting plasma glucose levels were nearly normalized within 48 hours and were less than 200 mg/dl for 11.7(]SY.+-。[)1.2 days in isografts and 17.8(]SY.+-。[)2.8 days in xenografts after transplantation. Mean K-values in isografts were 1.0 %/min at 2 weeks and 0.8 %/min at 4 weeks posttransplant, and those in xenografts were 1.3 %/min and 0.7 %/min at the same time points ; thus function of the bioartificial pancreas made of polysulfone gradually deteriorated within 1 month after transplantation, as fibrous tissue grew up around the fibers leading the inside of fibers to be hypoxic and to prevent diffusion of glucose and so forth. The effect of the device on carbohydrate metabolism was insufficient and transient, so that several modifications are required in order to apply it to clinical diabetics.
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