| Project/Area Number |
08671410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | UNIVERSITY OF TSUKUBA |
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Principal Investigator |
TODOROKI Takeshi UNIVERSITY OF TSUKUBA,SURGERY,ASSOCIATE PROFESSOR, 臨床医学系, 助教授 (70114105)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMOTO Toru UNIVERSITY OF TSUKUBA,SURGERY,ASSISTANT PROFESSOR, 臨床医学系, 講師 (30282354)
TAKADA Yasutsugu UNIVERSITY OF TSUKUBA,SURGERY,ASSISTANT PROFESSOR, 臨床医学系, 講師 (10272197)
HIRANO Takashi NATIONAL INSTITUTE OF BIOSCIENCES AND HUMAN TECHNOLOGY,HEAD, 生命工学研究所, 室長
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| Project Period (FY) |
1996 – 1998
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| Keywords | Hepatic reperfusion injury / SOD / Copolymer / SOD-DIVEMA conjugate / Hepatic microcirculation / DIVEMA / Leukocyte kinetics / Hepatic sinusoid |
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| Research Abstract |
A protective effects of the SOD (superoxide dismutase)-DIVEMA(divinyl ether and maleic anhydride) conjugate on hepatic ischemia-reperfusion injury was demonstrated. Twenty min. of normothermotic ischemia was induced by clamping the portal triad of Sprangue-Dawley rats. SOD, SOD-DIVEMA, or physiologic NaCl was administered 5 min. prior to reperfusion. Hepatic microcirculation was obserbed during the 120 min. reperfusion period using intravital-fluoresent microscope and events were'recorded to video system for the later analysis. SOD- DIVEMA restored the sinusoidal perfusion rate (98.5% for SOD-DIVEMA vs. 98.0% for no-ischemia, 65.5% for NaCl and 81.5% for SOD administration), and reduced the number of leukocyte stagnant in the acini (12.3 cells/lobule for SOD- DIVEMA vs. 4.4 for no-ischemia, 36.6 for NaCi, 27.7 for SOD administration) and adherent it in the postsinusoidal venule (40.0 cells/mm^2 for SOD-DWEMA vs. 55.0 for no-ischemia, 417 for NaCl and 253 for SOD administration) significantly in comparing control studies. Furthermore, SOD-DIVEMA maintained postischemic hepatocellular integrity . The SOD-DIVEMA treated group maintained significantly higher serum level of SOD activity after 120 min. of reperfusion than the SOD treated group ; 33.0 U/ml vs. 8.6 U/mi. The demonstration of the most beneficial efficacy of SOD-DIVEMA after the period of 120 min. of repercussion indicats a longer intravascular SOD activity than sole SOD administration.
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