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1997 Fiscal Year Final Research Report Summary

Study on cell proliferation in the gallbladder and bile duct epithelium with pancreaticobiliary maljinction.

Research Project

Project/Area Number 08671433
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionNagoya University

Principal Investigator

ANDO Hisami  Nagoya University, Department of General Medicine Assistant Professor, 医学部, 講師 (60184321)

Co-Investigator(Kenkyū-buntansha) KANEKO Ken-itiro  Nagoya University, Department of Pediatric Surgery Medical Doctor, 医学部, 医員
ITO Fujio  Nagoya University, Department of Pediatric Surgery Medical Doctor, 医学部, 医員
Project Period (FY) 1996 – 1997
Keywordspancreaticobiliary maljunction / choledochal cyst / PCNA / TGF-alpha / AgNOR / gallbladder / bile duct
Research Abstract

We have succeeded in making a new model for the pancreaticobiliary maljunction without bile duct dilatation, and demonstrated increased cell cycle of the gallbladder and the bile duct epithelium of this model.
A cat model was produced by performing choledocho-pancreatic side-to-side ductal anastomosis in nine animals. Six cats, in which the choledocho-pancreatic ducts were exposed only, served as controls. After 1 year, the common bile duct of these cats were removed and stained with anti-proliferating cell nuclear antigen (PCNA) antibody (PC10, Dako). The labeling index (LI) was determined from the percentage of positive nuclei in three microscopic fields.
Histologically periductal glands are well developed in models compared to controls. In the models, the number of PCNA positive cells was significantly increased at the connecting duct between the bile duct and glands. The mean ((]SY.+-。[) standard deviation) PCNA LI was 27.2 (]SY.+-。[) 10.9% in the models and 8.3 (]SY.+-。[) 4.2% in the controls (p<0.01). Histologically invaginations of the epithelium, sacculi of Beale increased in number and periductal glands were well developed. PCNA positive cells significantly increased in the common bile duct, especially in sacculi of Beale and glands.
These data clearly indicate that the cell cycle is increased not only in the gallbladder but also in the common bile duct and that excision of the bile duct is recommended in this disease.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hisami Ando: "Intrahepatic bile duct stenosis causing intrahepatic calculi formation following excision of a choledochal cyst." Journal of the American College of Surgeons. 183・1. 56-60 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisami Ando: "Complete excision of the intrahepatic portion of choledochal cysts." Journal of the American College of Surgeons. 183・4. 317-321 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenichiro Kaneko: "Increased cell proliferation and transforming growth factor-α(TGF-α)in the gallbladder epithelium of patients with pancreaticobiliary maljunction" Pathology International. 46.253-260 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenitiro Kaneko: "A new model for pancreaticobiliary maljunction without Dile duct dilatation:demonstration of cell proliferation in the gallbladder epithelium" Journal of Surgical Research. 60・1. 115-121 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisami Ando: "Operative treatment of congenital stenoses of the intrahepatic bile ducts in patients with choledochal cysts" The American Journal of Surgery. 173・6. 491-494 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenitiro Kaneko: "Protein plugs cause symptoms in patients with choledochal cysts" American Jornal of Gastroenterology. 92・6. 1018-1021 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 安藤 久實: "膵・胆管合流異常" 医学図書出版 船曳孝彦編集, 425 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 金子 健一朗: "膵・胆管合流異常" 医学図書出版 船曳孝彦編集, 425 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisami Ando, Kenitiro Kaneko, Takahiko Seo, Fujio Ito, Takahiro Ito: "Intrahepatic bile duct stenosis causing intrahepatic calculi formation following excision of a choledochal cyst." J.Am.Coll.Surg.183 (1). 56-60 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisami Ando, Kenitiro Kaneko, Takahiro Ito, Yoshio Watanabe, Takahiko Seo, Toru Harada, Fujio Ito, Masahiro Nagaya, Tetuyuki Sugito: "Complete excision of the intrahepatic portioon of choledochal cysts." J.Am.Coll.Surg.183 (4). 317-321 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenitiro Kaneko, Hisami Ando, Takahiro Ito, Yoshio Watanabe, Takahiko Seo: "Incresed cell proliferation and transforming growth factor-alpha (TGF-alpha) in the gallbladder epitherium of patients with pancreaticobiliary maljunction." Pathol.Int.46 (3). 253-260 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenitiro Kaneko, Hisami Ando, Takashi Umeda, Osamu Murahashi, Katumasa Hiraiwa, Norihiro Niimi, Moazzem Hossain, Takahiro Ito: "A new model for pancreaticobiliary maljunction without bile duct dilatation : demonstration of cell proliferation in the gallbladder epithelium." J.Surg.Resurch.60 (1). 115-121 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisami Ando, Kenitiro Kaneko, Fujio Ito, Takahiko Seo, Takahiro Ito: "Operative treatment of congenital stenoses of the intrahepatic bile ducts in patients with choledochal cysts" Am.J.Surg.173 (6). 4916-494 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenitiro Kaneko, Hisami Ando, Takahiro Ito, Yoshio Watanabe, Takahiko Seo, Toru Harada: "Protein plugs cause symptoms in patients with choledochal cysts." Am.J.Gastroenterol.92 (6). 1018-1021 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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