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1997 Fiscal Year Final Research Report Summary

INVOLVEMENT OF APOPTOSIS IN ORGAN FAILURE WITH SEVER ACUTE PANCREATITIS

Research Project

Project/Area Number 08671441
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKOBE UNIVERSITY

Principal Investigator

TAKEYAMA Yoshifumi  KOBE UNIVERSITY SCHOOL OF MEDICINE,FIRST DEPARTMENT OF SURGERY,ASSISTANT PROFESSOR, 医学部, 助手 (70263374)

Co-Investigator(Kenkyū-buntansha) HORI Yuichi  AFFILIATE HOSPITAL OF KOBE UNIVERSITY SCHOOL OF MEDICINE,FIRST DEPARTMENT OF SUR, 医学部・附属病院, 医員
UEDA Takashi  AFFILIATE HOSPITAL OF KOBE UNIVERSITY SCHOOL OF MEDICINE,FIRST DEPARTMENT OF SUR, 医学部・附属病院, 医員
Project Period (FY) 1996 – 1997
KeywordsAcute pancreatitis / Apoptosis / Organ failure
Research Abstract

The mechanism for organ failure in acute pancreatitis has not been clarified. Recently, apoptosis have been reported in organ failures with various conditions. Thus we hypothesized that apoptosis is involved in organ failures with severe acute pancreatitis. Necrotizing hemorrhagic pncreatitis was induced on rats by retrograde injection of sodium deoxycholate into pancreatic duct. Pancreatitis-associated ascitic fluid, which was collected 6 hours after development of pancreatitis, was injected intraperitoneally into untreated rats. Apoptosis was surveyed on the liver, kidney, and thymus from the rats histochemically by in situ nick-end labeling method and biochemically by DNA electrophoresis. In vitro, rat primary culture hepatocytes, isolated rat renal tubules, and normal rat kidney cell line (MDCK cells) were used, and apoptosis-inducing ability of the ascitic fluid was analyzed by nuclear staining and cell cycle analysis in addition to DNA electrophoresis.
As results, the hepatocytes, … More the renal tubules, and the thymocytes were labeled with in situ nick-end labeling in the rats with necrotizing hemorrhagic pancreatitis. Evident DNA fragmentations were detected in the liver, kidney, and thymus in the rats with pancreatitis. In addition, the hepatocytes and the renal tubules were labeled with in situ nick-end labeling in the rats with intraperitoneal injection of the ascitic fluid. In vitro analyzes, the ascitic fluid reduced cellular viabilities of the hepatocytes and MDCK cells in dose- and time-dependent manners. The ascitic fluid increased the ratios of apoptotic cells of the hepatocytes and MDCK cells in cell cycle analysis. Morphological analysis using nuclear staining fluolescent dye and DNA electrophoresis also indicated that the ascites induced apoptotoc cell death on the hepatocytes, isolated rat renal tubules, and MDCK cells.
In conclusion, apoptotic cell death occurs on liver, kidney, and thymus with severe acute pancreatitis within several hours, and is possibly involved in the mechanism of organ failure through underfined substance (s) in the ascitic fluid associated with severe acute pancreatitis. Less

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 竹山 宜典, 西川 淳介, 他: "重症急性膵炎における臓器障害と細胞死" 侵襲と免疫. 5・4. 31-34 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西川 淳介, 竹山 宜典: "急性膵炎における臓器障害とアポトーシス" 胆と膵. 18・1. 41-44 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 西川 淳介, 竹山 宜典: "重症急性膵炎における多臓器障害とアポトーシス" 外科治療. 77・1. 1-7 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹山 宜典, 山本 正博: "急性膵炎の重症化とアポトーシス" 消化器外科. 20・5. 617-623 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 新海 政幸, 竹山 宜典, 他: "Inhibitory Action of Sphingosine or Ceramide on Amylase Secretion from Isolated Rat Pancreatic Acini" Biochem Biophys Res Commun. 235. 197-200 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 正博, 竹山 宜典, 他: "臨床侵襲学" 小川道雄,斎藤英昭 編(へるす出版), 680 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshifumi Takeyama, Junsuke Nishikawa, et al.: "Ceal Death in Organ Failure with Severe Acute Pancreatitis" Surgical Trauma & Immunological Responces. 5 (4). 31-34 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junsuke Nishikawa, Yoshifumi Takeyama, et al.: "Organ Failure and Apoptosis in Acute Pancreatitis" Tan to Sui. 18 (1). 41-44 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Junsuke Nishikawa, Yoshifumi Takeyama, et al.: "Multiple Organ Failure and Apoptosis in Severe Acute Pancreatitis" Gekachiryo. 77 (1). 1-7 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Takeyama, Junsuke Nishikawa, et al.: "Involvement of Apoptotic cell death in Exacervation of Acute Pancreatitis" Shoukakigeka. 20 (5). 617-623 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masayuki Shinkai, Yoshifumi Takeyama, et al.: "Inhibitory Action of Sphingosine or Ceramide on Amylase Secretion from Isolated Rat Pancreatic Acini" Biochem Biophys Res Commun. 235. 197-200 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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