1997 Fiscal Year Final Research Report Summary
The prognosic value of the transcription of tumor growth-related genes in endoscopic biopsy specimens from patients with malignancies of digestive tracts
| Project/Area Number |
08671460
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Saga Medical School |
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Principal Investigator |
KATANO Mitsuo Saga Medical School Department of Surgery Assistant Professor, 医学部, 講師 (10145203)
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| Project Period (FY) |
1996 – 1997
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| Keywords | biopsy specimens / gastric carcinoma / mRNA / molecular and biological analysis / RT-PCR / survival time / prognostic factor / multivariate analysis |
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| Research Abstract |
The tumor biopsy specimens were obtained during preoperative endoscopy of patients with gastric carcinoma. The total RNA from each biopsy specimen was isolated and a reverse trascriptase-polymerase chain reaction analysis (RT-PCR) was used to analyze the transcription of tumor growth-related genes. The factors examined included cyclin D1 and cyclin E as cell cycle-related factors, urokinase-type plasminogen activator and 72-kd type IV collagenase as invasion-related ones, vascular endothelial growth factor and platelet-derived growth factor-A as angiogenesis-related ones, and transforming growth factor b and interleukin-10 as immunosuppression-related ones.
Results :
(1) The incidence of PDGF-A and TGF-b mRNA expression was preoperative indicator of lymph node involvement in early and advanced gastric cartcinomas, respctively.
(2) PDGF-A and TGF-b were preoperative prognostic indicators.
(3) Preoperative evaluation of malignant potential of each carcinoma may lead to a more reasonable classification for gastric carcinomas.
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