1998 Fiscal Year Final Research Report Summary
Diagnosis of microscopic peitoneal dissemination and cell damage and apoptosis
| Project/Area Number |
08671465
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
IMADA Toshio Yokohama City University, Associte Professor, 医学部・病院, 助教授 (50168514)
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| Co-Investigator(Kenkyū-buntansha) |
RINO Yasushi Yokohama City University, Senior Researcher, 医学部, 助手 (50254206)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Gastric cancer / Peritoneal dissemination / Cytology / Apoptosis / Caffeine / CDDP / 5-FU / CEA |
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| Research Abstract |
1. Dignosis of microscopic peritoneal dissemination in gastric cancer
(1) Conventional lavage cytology
Intraoperative lavage cytology was performed in 171 patients with serosal involvement, The positive rate was 29.2%. Multivariate analysis revealed that lavage cytology was the most important prognostic factor for gastric carcinoma.
(2) Detection of microscopic cancer cells by lavage cytology using immunostaining
Lavage cytology was examined by immunostaining using CEA, CA19-9, STN, SLX in 51 advance gastric cancer patients. Immunostaining was useful for comfirmation of cancer cell when conventional cytology yielded ambiguous results.
(3) Detection of microscopic cancer cells in lavage fluid by amplication of CEA mRNA
The expression of CEA mRNA was studied in 25 gastric cancer patients. Eleven of 25 lavage samples were involved by cancer cells by conventional cytology and all of these yielded the expected product by RT-PCR.Of the remaining 14 negative cytology samples. CEA mRNA was detected in 2 samples by RT-PCR.This method may lead to an early diagnosis of microscopic dissemination.
2. The effect of combination chemotherapy on gastric cancer cells and apoptosis
(1) Effect of combination of 5-FU and CDDP on the proliferation and morphology
The combination effect of 5-FU and CDDP was examined in terms of the prolieration, morphology. The cell growth inhibitory activity of 5-FU and CDDP against cancer cells was potentiated by the combined treatment with 5-FU and CDDP simultaneously, but not with CDDP followed by 5-FU.
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