1998 Fiscal Year Final Research Report Summary
Experimental therapeutics for gastric cancer using adenovirus vectors
| Project/Area Number |
08671476
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | NARA MEDICAL UNIVERSITY |
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Principal Investigator |
YAMADA Yukishige Nara Medical University, First Departmentof Surgery, Research Associate, 医学部, 助手 (50254496)
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| Co-Investigator(Kenkyū-buntansha) |
SAWADA Hidetomo Nara Medical University, First Departmentof Surgery, Assistant professor, 医学部, 講師 (10206021)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Gastric Cancer / Adenovirus Vector |
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| Research Abstract |
1) EGER and c-met expression after EGFR and c-met antisense expressing adenovirus in gastric cancer cells
Following infection with EGFR or c-met antisense RNA-expressing adenovirus(Ad-EAS or Ad-MAS), the cell surface EGER or c-met protein levels of infected cancer cell were observed by immunocytological method resulting in that markedly reduction of these proteins respectively. These suppression was not observed in non-infection or beta-galactosidase expressing adenovirus(Ad-GAL) infected group.
2) The effect of Ad-EAS virus on the growth of gastric cancer cells in vitro and in vivo.
Following infection with EGFR antisense RNA-expressing adenovirus(Ad-EAS), the in vitro growth of Ad-EAS infected cells was significantly inhibited relative to control infected cells in 3 gastric cancer cell lines (AGS, KKLS, MKN28) studied here(p(]SY.di-substituted right.[)O.0002). In a nude mouse subcutaneous tumor system, in vivo tumor growth of MKN28 was significantly inhibited after Ad-EAS treatment, and the inhibition on the 48th day was 93% by volume compared to that of untreated controls.
3) The effect of Ad-MAS virus on cancer cell motility in gastric cancer cells.
After Ad-MAS virus infection, the cell motility of AGS (c-met expressing) and Hs746t (c-met overexpressing) were significantly inhibited in comparison with control groups. The inhibition rate compare to non-infection group were 88% in AGS and 45% in Hs746t, respectively. These motility inhibition was not observed in KKLS cell (c-met non-expressing).
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