1997 Fiscal Year Final Research Report Summary
Role of Helicobacter pylori infection in perforation of peptic ulcer
| Project/Area Number |
08671489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
MATSUKURA Norio Dept.of Surg., Nippon Medical School, Assistant Prof., 医学部, 講師 (70124427)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Peptic ulcer / Helicobacter pylori / Perforation / Cytotoxin gene / cagA / vacA / case-control study / etiology |
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| Research Abstract |
Evidence showed a marked decrease in recurrence rate of peptic ulcer following eradication of Helicobacter pylori infection. However, whether H.pylori infection is etiologically related to perforation of peptic ulcer remains to be clarified. We therefore conducted and age and gender matched case-control study between perforated and non-surgical peptic ulcers in H.pylori infection and examined differences in cytotoxin genes, cag A and vac A.Serum H.pylori IgG antibody (ELISA) was positive in 20/21 (95%) of perforated vs. 37/40 (93%) of non-surgical duodenal ulcer and 5/5 (100%) of perforated vs. 24/28 (86%) of non-surgical gastric ulcer patients. Positivity of H.pylori DNA in gastric juice, which was amplified by PCR and identified by Southern blot hybridization was 17/23 (74%) of perforated vs. 32/45 (71%) in the non-surgical duodenal ulcer group. Positivity of cytotoxin genes, cagA and vacA ; in H.pylori DNA-positive gastric juice was as follows : perforated vs. non-surgical duodenal ulcer, cagA : 11/13 (85%) vs. 24/27 (89%) ; vacA1 : 9/13 (69%) vs. 22/27 (82%) ; vacA2 8/13(62%) vs. 21/27 (78%). There was no significant differences between the perforated and non-surgical peptic ulcers groups for these H.pylori serum and gene markers. It is assumed that H.pylori infection is not etiologically related to perforation of peptic ulcer.
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[Publications] Matsukura N., et al: Anatomy, Histology, Ultrastructure, Stomach, Rat."Monographs on Pathology of Laboratory Animals, 2^<nd> edition". (Jones, TC,Popp, JA,Mohr, U.eds) Springer-Verlag (Berlin, Heidelberg, New York), 343-450 (1997)
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