1997 Fiscal Year Final Research Report Summary
Inductions of myocardial 3-L-Nitrotyrosincin rat cardiac allograft rejection
| Project/Area Number |
08671505
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
OHMI Mikio Dept.Thorac & Cardiovasc Surg.School of Medcine, Associate Prof, 医学部・附属病院, 助教授 (00144931)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SADAHIRO Mituaki School surg.Lecturer, 医学部・附属病院, 講師 (80250778)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Keywords | cardiactransplantation / acuterejection / 3-L-nitrotyrosine |
|---|
| Research Abstract |
Heterotopic abdominal transplantation from Lewis to Fisher rats was used as a model of cardiac allograft rejection. Hearts were immediately removed after reperfusion used as sham control. Rats were sacrificed at 1,3, and 7 days after transplantation (n = 5 at each time point). Syngeneic Lewis to Liews abdominal heart transplant were also performed by the same technique (n = 3 at 1 and 7days). HPLC analysis, immunohistochemistry for nitrotyrosine, and histological studies were performed at each time points. N0_2-Tyr was induced with significant increases by day I compared with sham control (0.049*036% vs 0.000*0.000%, N0_2-Try/Total tyrosine, p<0.001) and continued to increase progressively through days 3(0.1060.011%, p <0.001) and 7 (0.162*0.013%, p <0.001). N02-Tyr immunostainina 01 infiltrating macrophages and cardiac muscle fibers increased significantly in the allograft at days 3 to 7. These results suggested that protein tyrosine nitration by NO plays a role in cardiac allograft rejection after transplantation.
|
Research Products
(2 results)
