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1997 Fiscal Year Final Research Report Summary

Analysis of mutation in the neurofiblomatosis type 2 (NF2) gene in preneoplastic and neoplastic lesion of human pleura.

Research Project

Project/Area Number 08671514
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionChiba University

Principal Investigator

SHIBA Mitsutoshi  Chiba University School of Medicine, Lecturer, 医学部, 講師 (20162620)

Co-Investigator(Kenkyū-buntansha) BABA Masayuki  Chiba University School of Medicine, Lecturer, 医学部・付属病院, 講師 (00143305)
SAITOH Yukio  Chiba University School of Medicine, Assistant, 医学部・付属病院, 助手 (60261905)
IIZASA Toshihiko  Chiba University School of Medicine, Assistant, 医学部, 助手 (10272303)
FUJISAWA Takehiko  Chiba University School of Medicine, Professor, 医学部, 教授 (80110328)
Project Period (FY) 1996 – 1997
Keywordsmalignant methothelioma / NF2 gene / gene therapy / PCR-SSCP / Immunohistochemistry / malignant pleurisy of lung cancer / Ki-67 antigen
Research Abstract

(Background and aim) It was reported high incidence of mutation in the neurofiblomatosis type 2 (NF2) gene in human malignant methothelioma. To establish a model of gene therapy with NF2 gene for malignant methothelioma, we performed genetic analysis of NF2 gene with DNA of malignant methothelioma and tumor adjacent pleura of surgically resected malignant methothelioma cases. And we also performed immunohistochemical analysis of these malignant methothelioma and malignant pleurisy cases of lung cancer to evaluate mutation of NF2 gene and biological behavior of the tumor. (Materials and Methods) Three cases of resected malignant methothelioma and 65 cases of resected lung cancer cases with subclinical pleural dissemination were examined. Tumor DNA was isolated by phenol-chloroform method and mutation of NF2 gene was evaluated by PCR-SSCP method. Immunohistochemistry was performed with C-18 and A-19 antibody for NF2 gene, MIB-1 antibody for Ki-67 antigen and DO-7 antibody for p53 oncoprotain. (Results and Conclusion) In these three cases, genomic PCR amplification was successfully obtained in the exon 3, exon 4, exon 7, exon 13 and exon 16 of NF2 gene. But no apparent mutation of NF2 gene was detected by SSCP analysis which was performed in these five exons. Immunohistochemical analysis also showed the same results with C-18 and A-19 antibodies. Ki-67 labeling index was low and negative for p53 oncoprotain in two methothelioma cases. In lung cancercases, it was disclosed Ki-67 labeling index was independent prognostic factor and correlated well with clinical course of the lung cancer cases with pleural dissemination. Further investigation with more methothelioma cases must be needed to evaluate the role of NF2 gene in malignant methothelioma patients.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 光永 伸一郎 他: "ヒト悪性中皮腫における2型神経線維腫症遺伝子の突然変異" 肺癌. 35. 567-567 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柴光 年 他: "中枢気道に発生した腺〓嚢包癌切除例の臨床的検討" 肺癌. 36. 117-124 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柴光 年 他: "開胸時悪性胸水が発見された肺癌に対する外科治療成績の検討" 日本外科学会誌. 97. 77-77 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柴光 年 他: "III IV期浸潤型胸腺腫および胸腺癌に対する〓学的治療" 日本呼吸器外科学会誌. 11. 325-325 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松澤 公孝 他: "原発性肺非小細胞癌におけるp53遺伝子突然変異の検討" 肺癌. 37. 5-11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiba M.et al.: "Surgical treatment for carcinoma plearious found at thoratory in being cancer patoects" Journal of Thoracic and cardtvasc Surg.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsunaga S,Shiba M,Baba M,et al.: "Mutation of neurofiblomatosis type 2 (NF2) gene in human malignant methothelioma." Haigan (Japanese Journal of Lung Cancer). 35. 567-567 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiba M,Yamaguchi Y,Mitsunaga S,et al.: "Clinical study of adenoid cystic carcinoma originated in the central airway." Haigan (Japanese Journal of Lung Cancer). 36. 117-124 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiba M,Yamaguchi Y,Iizasa T,et al.: "Surgical therapy for lung cancer with pleural dissemination which was found at the time of thoracotomy." Japanese Journal of Surgery. 97. 77-77 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiba M,Yoshida S,Suzuki M,et al.: "Combined therapeutic modality for advanced invasive thymoma and thymic cancer." Journal of the Japanese Association for Chest Surgery. 11. 325-325 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kakizawa K,Yamaguchi Y,Shiba M,et al.: "p53 mutation in primary non-small cell lung carcinoma." Haigan (Japanese Journal of Lung Cancer). 37. 5-11 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiba M,Kakizawa K,Mitsunaga S,et al.: "Surgical treatment for carcinomatous pleuritis found at thoracotomy in lung cancer patients and prognostic significance of Ki-67 labeling index." J Thoracic Cardiovasc Surg. (submitted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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