1998 Fiscal Year Final Research Report Summary
Gene therapy for brain fumor using adenovirus-vector with integrinalpha_3
| Project/Area Number |
08671577
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
AOKI Tomokazu Kyoto Univ.Neurosurgey, Assistant Prof, 医学研究科, 助手 (50273454)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATAKE Shinichi Kyoto Univ.Neurosurgey, Associate Prof, 医学研究科, 講師 (90209916)
TAKAHASHI Juna Kyoto Univ.Neurosurgey, Assistant Prof, 医学研究科, 助手 (80252435)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Brain tumor / Integrin / monoclonal antibody / gene therapy |
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| Research Abstract |
We have established astrocytoma specific monoclonal antibody and have purified the protein which the monoclonal antboby recognizes. We have determined the amino acid alignment at N terminal of the protein by microseaquence method. It proved to be human integrin a3.
Human integrin a3 was strongly expressed at astrocytoma using Northern blotting, western blot and immunohistochemistry.
Our establishing monoclonal antibody inhibits the growth of glioma cell line and elicit the apoptpsis of it. DNA pattern showed a laddering.
A shorter band than the expected band of Integrina3 cDNA was appeared in northern blotting. That shorter band was correlated to the malignancy of glioma.
Splice variant was cloned by PCR and was sequenced. That result showed delatin of cytoplasmic region with phosphorilation site. This delation inhibits the signal transduction and disturbed the cell contact and resulted in invasion to normal brain.
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[Publications] Kojima, M., Hoshimaru, M., Aoki, T., Takahashi, J., T.Ohtsuka, Asahi, M., Matsumura, N., and Kikuchi, H.: "Expression of heat shock proteins in the developing rat retina." Neuroscience Letters. 205. 215-217 (1998)
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[Publications] Zhu, Z., Kimura, M., Itokawa, Y., Aoki, T., Takahashi, J., Nakatsu, S., Oda, Y., and Kikuchi, H.: "Apoptosis induced by selenium in human glioma cell lines" Biological Trace Element Reserch. 52. 1-15 (1996)
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[Publications] Asahi, M., Hoshimura, M., T.Ohtsuka, Asahi, M., Matsumura, N., Aoki, T., Shibahara, K., and Kikuchi, H: "Nedd-2 gene is induced during transient global ischemia in rats" Journal of Cerebral Blood Flow and Metabolism. 17. 11-18 (1997)
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[Publications] Takahashi, Jc, M,Saiki, S,Miyatake, S,Tani, H,Kubo, K,Goto, T,Aoki, JA,Takahashi, I,Nagata, H,Kikuchi: "Adenovirus-mediated gene transfer of basic fibroblast growth factor induces in vitro angiogenesis." Atherosclerosis. 132. 199-205 (1997)
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[Publications] Ikeda, A., S.Yazawa, T.Kunieda, K.Araki, T,Aoki, H.Hattori, W.Taki, and H.Shibasaki.: "Scalp-recorded, ictal focal DC shift in a patient with tonic seizure" epilepsia. 38(12). 1350-1354 (1997)
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[Publications] Saga, T., H.Sakahara, Z.Yao, Y.Nakamoto, N.Sato, N.Hattori, M.Zhang, S.Zhao, T.Aoki, S.Miyatake, Y.Namba, and J.Konishi: "detection of altered adhesion molecule expression in experimental tumors by a radiolabeled monoclonal antibody." Jpn.J.Cancer Res. 88. 1171-1180 (1997)
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[Publications] Tani, S., M.Taniwaki, Y.Taniguchi, S.Minoguchi, K.Kuroda, H.Han, T.Aoki, S.Miyatake, N.Hashimoto, T.Honjo: "chromosomal mapping of two RBP-J-related genes : Kyo-T and RBP-L." J.Hum.Genet.44. 73-75 (1999)
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[Publications] Tani, S., T.Aoki, A.Nakano, S.Miyatake, M.Saiki, Jc.Takahashi, H.Kikuchi, Y.Namba, T.Honjo: "The astrocyte-specific antibody, GA-17, recognizes human integrin alpha 3 subunit." (in submission).
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