| Co-Investigator(Kenkyū-buntansha) |
ARITA Norio Osaka Univ., Dept.of Neurosurg., Associated Professor, 医学部, 講師 (80159508)
HAYAKAWA Toru Osaka Univ., Dept.of Neurosurg., Professor, 医学部, 教授 (20135700)
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| Research Abstract |
To facilitate the regeneration of crush-injured facial nerve, purified Schwann cells were placed around the crush-injured nerve.
Mature Schwann cells were obtained from dorsal root ganglia of SD rats as described by Kim et al. The two peripheral branches of left facial nerve of each SD rat (10 weeks old) were exposed, and pinched with aneurysm clip (Sugita No.1) for 2 minutes. The evoked electromyograms were recorded by stimulating the proximal and distal portions of the exposed facial nerve (8mA constant current). The mean distance between the two stimulation points was 25mm, and nerve conduction velocity (NCV) was calculated before injury, just after injury, and 14 days after injury. Ten crush-injured portions were covered with collagen gel (0.3ml) containing Schwann cells (1.0*10<@D18@>D1/ml) (Schwann group), and the other 8 were covered with collagen gel only (control group). After electrophysiological studies, the rats were perfused with 4% paraformaldehyde, and the nerves were removed and embedded in paraffin. The 5 mum-thick slices were stained for HE and S-100 protein immunocytochemically. The baseline NCV was 47.6(]SY.+-。[)6.8m/s (mean(]SY.+-。[)SE), and it reduced remarkably just after injury (11.8(]SY.+-。[)2.7m/s). Fourteen days after injury, NCV of Schwann group (30.3(]SY.+-。[)8.6m/s) was significantly greater than that of control group (11.2(]SY.+-。[)3.6m/s). However, immunocytochemistry failed to reveal transplanted Schwann cells around crush-injured nerve.
From these results, transplantation of Schwann cells around crush-injured facial nerve may facilitate the nerve regeneration in the early period after crush-injury. However, it was difficult to determine the substance and mechanism of facilitation of the repair of injured nerves.
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