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1997 Fiscal Year Final Research Report Summary

Investigation About Molecular Biological Changes In Diffuse Axonal Injury Model And Therapeutic Possibility With Neurotrophic Factors

Research Project

Project/Area Number 08671580
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionOsaka University

Principal Investigator

KOHMURA Eiji  Osaka University Medical School, Assistant Professor, 医学部, 助手 (30225388)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Toru  Osaka University Medical School, Professor, 医学部, 教授 (20135700)
Project Period (FY) 1996 – 1997
Keywordsdiffuse axonal injury / head injury / neurotrophic factor / molecular biology
Research Abstract

An animal model of diffuse axonal injury was first produced. ASD rat was placed on urethane foam with a steel helmet on the exposed skull under general anesthesia. The helmet was hit by a free falling weight of 450g. The height was adjusted to 1.5 meter so that animal survival could be expected. Most animals presented with apnea and convulsion for a short period. Histological examination revealed that axonal retraction balls and swelled axons are produced in the basal pons till 2 weeks. We tried to find changes of mRNA coding receptors for neurotrophic factors but failed to detect them because there were variations of injury severity between animals and the sensitivity of detection method.
Next, we tried to find usefulness of minipellet containing BDNF (200mg). This minipellet can release BDNF continuously for about one week. We examined the effect on the model of facial never injury and intracerebral hematoma, because facial motoneurons and dopaminergic neurons of substantia nigra are known to respond against BDNF.Recovery of facial function was accelerated by BDNF minipellet. Tyrosine hydroxylase positive neurons were well preserved in the substantia nigra in the hematoma model by BDNF pellet. To apply trophic factors via minipellet may be clinically feasible. We should further study the precise mechanism of the action of trophic factors.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 湯口貴導: "中枢神経損傷後の神経保護、ならびに再生関連遺伝子の発現変化." 神経外傷. 19. 68-73 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita T: "Changes in glutamate/aspartate transporter (GLAST/GluT-1) mRNA expression following facial nerve transection." Mol Brain Res. 38. 294-299 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita T: "Induction of Na+/myo-inositol cotransporter mRNA after focal cerebral ischemia : Evidence for extensive osmotic stress in remote areas." J Cereb Blood Flow Metab. 16. 1203-1210 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohmura E: "Antisense oligonucleotide for heat shock protein increases loss of CA3 neurons in hippocampal slice." J Cereb Blod Flow Metab. 17. S488- (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohmura E: "Altered expression of Growth Inhibitorv Factor (GIF/MT-III) mRNA in the rat facial nucleus after facial nerve injury is closely related with facial function." Restorative Neurology and Neuroscience. 11. 169-175 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuguchi T: "Expression of tPA mRNA in the facial nucleus following facial nerve transection in the rat." Neuroreport. 8. 419-422 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita T: "Changes in glutamate/aspartate transporter (GLAST/GluT-1) mRNA expression following facial nerve transection." Mol Brain Res. 38. 294-299 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita T: "Induction of Na+/myo-inositol cotransporter mRNA after focal cerebral ischemia : Evidence for extensive osmotic stress in remote areas." J Cereb Blood Flow Matab. 16. 1203-1210 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohmura E: "Antisense oligonucleotide for heat shock protein increases loss of CA3 neurons in hippocampal slice." J Cereb Blood Flow Metab. 17. S488 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohmura E: "Altered expression of Growth Inhibitory Factor (GIF/MT-III) mRNA in the rat facial nucleus after facial nerve injury is closely related with facial function" Restorative Neurology and Neuroscience. 11. 169-175 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakaki T: "Loss and apoptosis of smooth muscle cells in intracranial aneurysms. Studies with in situ DNA end labeling and antibody against single-stranded DNA" Acta Neurochir. 139. 469-475 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuguchi T: "Expression of growth inhibitory factor mRNA after focal ischemia in rat brain." J Cereb Blood Flow Metab. 17. 745-752 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuguchi T: "Expression of tPA mRNA in the facial nucleus following facial nerve transection in the rat." Neuroreport. 8. 419-422 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada K: Ischemic neuronal injury and gene expression of facillitative and inhibitory growth factors. In "Maturation Phenomenon in Cerebral Ischemia II", U.Ito et al.(Eds), Springer-Verlag, Berlin Heidelberg, 27-32 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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