1997 Fiscal Year Final Research Report Summary
Investigation About Molecular Biological Changes In Diffuse Axonal Injury Model And Therapeutic Possibility With Neurotrophic Factors
| Project/Area Number |
08671580
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
KOHMURA Eiji Osaka University Medical School, Assistant Professor, 医学部, 助手 (30225388)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Toru Osaka University Medical School, Professor, 医学部, 教授 (20135700)
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| Project Period (FY) |
1996 – 1997
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| Keywords | diffuse axonal injury / head injury / neurotrophic factor / molecular biology |
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| Research Abstract |
An animal model of diffuse axonal injury was first produced. ASD rat was placed on urethane foam with a steel helmet on the exposed skull under general anesthesia. The helmet was hit by a free falling weight of 450g. The height was adjusted to 1.5 meter so that animal survival could be expected. Most animals presented with apnea and convulsion for a short period. Histological examination revealed that axonal retraction balls and swelled axons are produced in the basal pons till 2 weeks. We tried to find changes of mRNA coding receptors for neurotrophic factors but failed to detect them because there were variations of injury severity between animals and the sensitivity of detection method.
Next, we tried to find usefulness of minipellet containing BDNF (200mg). This minipellet can release BDNF continuously for about one week. We examined the effect on the model of facial never injury and intracerebral hematoma, because facial motoneurons and dopaminergic neurons of substantia nigra are known to respond against BDNF.Recovery of facial function was accelerated by BDNF minipellet. Tyrosine hydroxylase positive neurons were well preserved in the substantia nigra in the hematoma model by BDNF pellet. To apply trophic factors via minipellet may be clinically feasible. We should further study the precise mechanism of the action of trophic factors.
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