1997 Fiscal Year Final Research Report Summary
REGULATION OF CEREBROSPINAL FLUID CIRCULATION AND INTRACRANIAL PRESSURE BY WATER CHANNEL
| Project/Area Number |
08671600
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | NAGOYA CITY UNIVERSITY |
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Principal Investigator |
MASAGO Atsuo NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 助手 (70209419)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAMURA Yasuhiro NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 助手 (40295613)
KATANO Hiroyuki NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 助手 (30295612)
MASE Mitsuhito NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,INSTRUCTOR, 医学部, 助手 (60238920)
MATSUMOTO Takashi NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,ASSISTANT PROFESSOR, 医学部, 講師 (50199676)
YAMADA Kazuo NAGOYA CITY UNIVERSITY,MEDICAL SCHOOL,PROFESSOR, 医学部, 教授 (90150341)
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| Project Period (FY) |
1996 – 1997
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| Keywords | intracranial pressure / cerebrospinal fluid / hydrocephalus / osmolyte transporter / water channel |
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| Research Abstract |
The present study was designed to investigate molecular regulation of cerebrospinal fluid circulation under the high intracranial pressure. The investigators proposed research projects focused on the expression of water channel, osmolyte transporters and natriuretic peptide. The following results were obtained. (1)EXPRESSION OF WATER CHANNEL GENE : Water channel transports water molecules across the cell membrane into or out of the cells. Water channel is supposed to regulate water content in the brain tissue. Using in situ hybridization histochemistry, water channel (MIWC) mRNA was expressed in the ependymal cells and cerebral white matter. However, MIWC mRNA expression did not change in the high intracranial environment and kaoline-induced hydrocephalus in rats. Water channel includes other homologous superfamilies which might control water content in the pathological conditions. (2)C-TYPE NATRIURETIC PEPTIDE (CNP) : CNP is widely present over the central nervous system and plays a r
… More
ole in the hormonal regulation of systemic blood circulation. The expression of CNP mRNA was changeless after the development of hydrocephalus. The CNP gene might fluctuate within a short period and could not be evaluated satisfactorily. (3)GLUTAMATE/ASPARTATE TRANSPORTER (GLAST) : GLAST plays an important role in regulation of osmotic pressure and extracellular glutamate concentration. In the acute stage of experimental hydrocephalus, periventricular reactive astrocytes markedly expressed GLAST mRNA.High ICP and ventricular dilatation in the acute hydrocephalus cause periventricular edema and neuronal damage. GLAST gene might be induced in order to regulate local osmotic pressure or remove excess glutamate released from injured axons (Masago et al.1996). (4)SUBARACHNOID HEMORRHAGE (SAH) MODEL AND NEURONAL STRESS : We developed original SAH model in rats by endovascular perforation with nylon thread. This model represents closely aneurysmal SAH.We are investigating osmoregulation using this SAH model. Less
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