1997 Fiscal Year Final Research Report Summary
Experimental study of gene therapy for malignant glial cells by transfection with IL-12 gene -using SCID mouse model imitating human being-
| Project/Area Number |
08671609
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Jichi Medical School |
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Principal Investigator |
HASHIMOTO Masaaki JIchi Medical School, Neurosurgery, assistant professor, 医学部, 講師 (60221496)
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| Co-Investigator(Kenkyū-buntansha) |
HATAKE Kiyohiko Jichi Medical School, Hematology, associate professor, 医学部, 助教授 (80192699)
NAGAI Mutsumi Jichi Medical School, Neurosurgery, assistant, 医学部, 病院助手 (10265259)
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| Project Period (FY) |
1996 – 1997
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| Keywords | IL-12 gene / malignant glioma / SCID mouse / experimenta model imitating / human being / gene therapy / ex vivo |
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| Research Abstract |
1.Making an Experimental model imitating human being
After the intraperitoneal administration of SCID mouse with human mononuclear cells, these human mononuclear cells were detected until 3 weeks. Administration of human IL-2 simultaneously prolonged the life span of human mononuclear cells about 1 or 2 weeks. It suggests that human IL-2 activated human NK cells and T cells. We don't know why some SCID mouse died after the administration early. So it is necessary for experimental model to use the other cytokines.
2.IL-12 expression in patients with malignant glioma, having beta-IFN therapy
For patients with malignant astrocytoma or glioblastoma, we underwent immunotherapy with beta-IFN (3 million unit) every other day. During the therapy, NK activity, LAK activity and IL-12 expression were measured. However we cannot find NK activity, LAK activity and IL-12 expresion significantly.
3.Transfection of IL-12 gene (p35, p40) into malignant glioma cell line
IL-12 gene could not be successfully introdued into malignant glioma cell line. Both subunits of p35 and p40 are nessesary for active type of IL-12. So we did double transfection of P35 and p40 with electroporation. Now we investigate to find the surest way of induction of IL-12. On the other hand, in vitro study IL-12 induced a considerable antitumor effect from human mononuclear cells on human glioma cell line, even compared with IL-2. However, for the purpose of clinical trial of IL-12, it is important to establish the ex vivo model.
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