1998 Fiscal Year Final Research Report Summary
Etiological study of the ossification of the spinal ligament.-The immunological and endocrinological studies about general ossification-
| Project/Area Number |
08671697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Department of Orthopaedic Surgery, Toho University School of Medicine |
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Principal Investigator |
OKAJIMA Yukikazu Department of Orthopaedic Surgery, Toho University School of Medicine Professor, 医学部, 教授 (60104212)
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| Co-Investigator(Kenkyū-buntansha) |
KAKIUCHI Terutaka Department of Orthopaedic Surgery, Toho University School of Medicine Professor, 医学部, 教授 (40126024)
OKADA Yayoi Department of Orthopaedic Surgery, Toho University School of Medicine Assistant, 医学部, 助手 (60256758)
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| Project Period (FY) |
1996 – 1998
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| Keywords | OPLL / Estrogen / Cultured cell / Cell growth factor / Cytokine / Mononucleocyte / T cell function / CD4 positive T cell |
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| Research Abstract |
We investigated the effect of estrogen as a potential causative for ossification of the posterior longitudinalligament (OPLL). The serum estrogen level was significantly higher in the OPLL patients than controls. Cultured cells derived from OPLL patients had receptors with a higher affinity for estradiol than cells from controls. Cultured cells derived from OPLL patients responded to stimulation by estradiol, elevated the tritiated thymidine uptake and increased the BGP products.
In the study of cell growth factors (TGF beta -1, b-FGF), cultured cells derived from OPLL patients stimulated by estradiol, it was found that productive ratio of TGF beta-1 and b-FGF was increased.
We also studied that proliferative response of peripheral mononuclear cell (PMNC). When PMNC was stimulated by mitogens such as phytohemagglutinin (PHA), pokeweed mitogen (PWM), Staphylococcus aureus Cowan I (SACI), and monoclonal antibody such as antiCD3 mAb, the prolifetative response of PMNC from OPLL patients was lower than that from healthy controls. IL-2 production of T cells stimulated by PHA or antiCD3 mAb was lower in OPLL patients than healthy controls.
And then PMNC stimulated by PHA or anti CD3 mAb with or without TGF beta-1 or b-FGF.Both TGF beta-1 and b-FGF, in addition of higher concentration, it found that the proliferative response was suppressed.
It was studied that proliferative response of CD4 T cells purified from PMNC to anti-CD3 mAb is lower in OPLL patients than in healthy controls, too. And IL-2 production of CD4 T cells was also lower in OPLL patients than in healthy controls.
In the Study of cell surface markers, CD3, CD4, CD8, CD19, CD25 were detected by flow cytometry. It was not found between OPLL group and healthy controls that difference about subpopulation of lymphocyte.
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