1997 Fiscal Year Final Research Report Summary
Dynorphin mRNA expression in dorsal horn neurons following traumatic spinal cord injury and the effect of neurotrophic factor
| Project/Area Number |
08671700
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | HYOGO COLLEGE OF MEDICINE |
|---|
Principal Investigator |
TANIGUCHI Mutusmi HYOGO COLLEGE OF MEDICINE,Assitant Professor, 医学部, 講師 (80188383)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Koichi Professor, 医学部, 教授 (10212127)
MARUO Soji Professor, 医学部, 教授 (70068451)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Keywords | spinal cord injury / dynorphin / in situ hybridization / neurotrophic factor |
|---|
| Research Abstract |
The temporal and spatial distribution of preprodynorphin (PPD) mRNA expression in the injured rat spinal cord was examined by in situ hybridization. Rats were subjected to traumatic spinal cord injury at the T13 spinal segment using the weight drop method. Two double-labeling experiments revealed that increased PPD mRNA and dynorphin peptide expression were found exclusively in dorsal horn neurons. Neurons exhibiting an increase in the level of PPD mRNA were concentrated in the superficial laminae and the neck of dorsal horn within several spinal segments from the epicenter of the injury at 24h and 48h after injury. The number of neurons expressing PPD mRNA in each rat was significantly positively correlated with its motor dysfunction. These data supports the hypothesis that the dynorphin has a pathological role in secondary tissue damage and neurological dysfunction after spinal cord injury.
|
