1998 Fiscal Year Final Research Report Summary
The role of calcitriol in the immune system
| Project/Area Number |
08671823
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | University of Occupational and Environmental Health (1998)
Kyushu University (1996-1997) |
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Principal Investigator |
TAKAHASHI Kouichi University of Occupational and Environmental Health, Department of Urology, Assistant professor, 医学部・泌尿器科, 講師 (30284491)
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| Co-Investigator(Kenkyū-buntansha) |
UOZUMI Jiro Kyushu University, Department of Urology, Assistant professor, 医学部・泌尿器科, 講師 (30223514)
TOKUDA Noriaki Kyushu University, Department of Urology, Assistant professor, 医学部・泌尿器科, 助手 (20264038)
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| Project Period (FY) |
1996 – 1998
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| Keywords | calcitriol / 22-oxa-calcitriol / Prostate cancer / Renal cancer |
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| Research Abstract |
1. Calcitriol(1,25D) roles in hemodialysis patients(HDP).
In vitro I , 25D treatment markedly reduced HLA-DR(DR) and stimulated phagocytosis in peripheral blood monocytes(PBM) from both 19 HDP and 9 normal subjects(NS).
DR by monocytes and the phorbol myristate acetate(PMA)-induced superoxide production(SOP) by neutrophils were significantly higher in HDP than in NS.The phagocytosis in HDP decreased significantly more than that in NS.Moreover, the mitogen responses(MR) by peripheral blood lymphocytes also decreased significantly against PWM and insignificantly against Con A.In vivo I , 25D treatment resulted in a marked decrease in DR by PBM in HDS.Moreover, the treatment significantly enhanced the PMA-induced SOP.However, the phagocytosis by neutrophils and the MR to Con A and PWM by lymphocytes were not significantly affected by this treatment.
2.1,25D effect in urologic malignancies.
(1)A dose-dependent anti-proliferative effect of 1,25D was found in prostate cancer cell lines, PC-3, PPC- 1 and ALVA-lOl and not in ALVA-41. HLA-ABC(ABC) was expressed in PC-3, ALVA-41 and ALVA-lOl, but not in PPC-1. These expressions were either enhanced or induced by interferon-gamma (IFN-gamma). DR was not expressed and also not induced by IFN-gamma. ICAM-l was expressed in all cells and slightly up-regulated by IFN-gamma. There were no detectable effects of 1,25D on ABC, DR and ICAM-1.
(2)Any anti-proliferative effect of 1,25D was not found in human renal cancer cell lines, KPK-l and SNl2C.ABC was expressed and enhanced by IFN-gamma. DR was not expressed in these cells and induced by LFN-gamma only in KPK-1 cells. There were no detectable effects of 1,25D on both ABC and DR.
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