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1997 Fiscal Year Final Research Report Summary

In vivo gene transfer methods into bladder without viral vectors

Research Project

Project/Area Number 08671833
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

KISHIMOTO Taketoshi  Osaka City Univ.Med.School, (Dept.Urology) Professor, 医学部, 教授 (00047078)

Co-Investigator(Kenkyū-buntansha) WADA Seiji  Osaka City Univ.Med.School, (Dept. Urology), Assistant Professor, 医学部, 講師 (20158695)
SUGIMURA Kazunobu  Osaka CIty Univ.Med.School, (Dept.Uroligy), Assistant Professor, 医学部, 講師 (90187659)
Project Period (FY) 1996 – 1997
KeywordsGene transfer / Bladder / HVJ-liposome / Particle gun / Electroporation
Research Abstract

objective GEne therapy has been adapted as a new strategy for treating cancers. When considering this application to bladder cancer, safe, efficient and repeatable in vivo geno transfer metods are essential. Here, we examined three in vivo gene transfer methods without viral vectors.
Materials and methods Hemagglutinating virus of Japan (HVJ)-liposomes possessing the membrane fusion activity were intraluminally injected into rat bladders. Using a particle gun, rabbit bladder mucosa was bombarded with DNA-Coated gold microcarriers. Electrotransfection was also examined in rabbit bladder by pulse direct currents (0.15-0.2 A, 50msec, repeated 8 times) generated between needle electrodes after submucous injection of DNA solution. beta-galactosidase gene and chloramphenicol acetyl-transferase (CAT) gene were used as marker genes.
Results HVJ-liposomes efficiently transfected superficial layrs of urothelium with a peak of expression on day 5. The particle gun produced non-uniform but efficient transfection in deeper layrs of the urothelim. By electransfection, submucous interstitial cells were transfected as well as urothelim. No major complications were observed in these three metheds.
Conclusion HVJ-liposomes are potentially useful for treating carcinoma in situ. With further refinement, the latter two methods may be suitable for adjuvant therapies in treating localized bladder tumors.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 杉村 一誠 他: "非ウイルス・ベクターによる膀胱へのin vivo遺伝子導入法" 泌尿器科紀要. 43・11. 823-827 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harimoto K, et al.: "In vivo gene transfer methods into bladder without viral vectors" British Journal of Urology. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazunobu Sugimura, et al.: "In vivo gene transfer methods into bladder without viral vectors" Acta Urol Jpn. 43 (11). 823-827 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kouji Harimoto, et al.: "In vivo gene transfer methods into bladder without viral vectors" Brit J Urol. (in Press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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