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1997 Fiscal Year Final Research Report Summary

Analysis of the oxytocin unresponsiveness due to the mutant oxytocin receptor.

Research Project

Project/Area Number 08671889
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionOsaka University

Principal Investigator

TOKUGAWA Yoshihiro  Osaka University Medical School, Assistant Professor, 医学部, 助手 (70283786)

Co-Investigator(Kenkyū-buntansha) SIMOYA Kouitchirou  Osaka University Medical School, Assistant Professor, 医学部, 助手 (40291950)
AZUMA Chihiro  Osaka University Medical School, Lecturer, 医学部, 講師 (20151061)
SAJI Fumitaka  Osaka University Medical School, Associate Professor, 医学部, 助教授 (30203897)
Project Period (FY) 1996 – 1997
Keywordsoxytocin unresponsiveness / oxytocin binding capacity / N-glycosylation / regulatory element / 発現調節領域 / 糖鎖
Research Abstract

1. We have established permnent cell-lines in which the gene for the human oxytocin receptor has been introduced into HeLa cells. These cells differ by the disruption of hte N-terminal N-glycosylation sites by site-directed mutagenesis of the transfected oxytocin receptor constructs. The mutation of N-glycosylation sites appear to affect neither their dissociation constant, nor affinites for various oxytocin related ligands. This suggests that the full glycosylation of oxytocin receptor observed in vivo is not essential for its activity.
2. We examined the localization and expression of the oxytocin receptor in human myometrium by means of in situ hybridization, immunohistochemistory, and Northern and Western blotting. In the term pregnant myometrium, oxytocin receptor was expressed in uterine smmoth muscles. The level of oxytocin receptor transcripts increased according to the course of pregnancy. We also found that the receptor protein was augmented at term and after the onset of labor.
3. We determined the nucleotide sequence of the 5'-flanking region of the mouse oxytocin receptor gene, and compared the sequence with human, rat, and bovine sequence. This comparison revealed that a region commencing at nt-1671 to -1581 was a clear homology among the species. This sequence information will help to characterize the regulatory components of oxytocin receptor.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] T.Kimura, et al: "The role of N-terminal glycosylation in the human oxytocin receptor." Molecular Human Reproduction. 3.(11). 957-963 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Simoya, et al: "Regulation of placental monocyte chemotactic and activating factor during pregnancy and chorioamnionitis" Molecular Human Reproduction. 4.(2). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Tokugawa, et al: "Lipocalin-Type Prostaglandin D Synthase in Human Male Reproductive Organs and Seminal Plasma" BIOLOGY OF REPRODUCTION. 58. 600-607 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Simoya, et al: "Interleukin-8 level in maternal serum as a maker for screening of histological chorioamnionitis at term" International Journal of Gynecology & Obstetrics. 57. 153-159 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Hasimoto, et al: "BIPARENATAL ALLELES OF HLA-G ARE CO-DOMINANTLY EXPRESSED IN THE PLACENTA" Jpn J Human Genet. 42. 181-186 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kanai, et al: "Increased Interleukin-1 and Interleukin-1 Receptor Antagonist Lvels in Cervical Mucus in the Ovulatory Phase in Comparison with the Follicular Phase" Gynecologic and Obstetric Investigation. 43. 166-170 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Shimoya, et al: "Effect of sexual intercourse on fetal fibronectin concentration in cervicovaginal secretions" American journal Obstet Gynecol. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より

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Published: 1999-03-16  

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