1997 Fiscal Year Final Research Report Summary
Interphase cytogenetic study of apoptosis-related chromosomes and genes
| Project/Area Number |
08671918
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Obstetrics and gynecology
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
MATSUTA Morimasa IWate Med.Uni.Dept of OB/GYN,associated Prof Pso, 医学部, 講師 (40157326)
|
|---|
| Project Period (FY) |
1996 – 1997
|
| Keywords | Apoptosis / In situ hybridization / Chromosome |
|---|
| Research Abstract |
It was clear that apoptosis plays an important role in cytotoxic mechanisms by anticancer agents. It must be the best way to observe either genetic or morphological changes, when the genetic aberrations during the induction and development of apoptosis are analyzed. We planed the interphase cytogenetic study using Laser Scanning Cytometer (LSC) and Fluorescence In situ Hybridization (FISH). Apoptotic cells are easily recognized morphologically by LSC,and histogram of these cells are analyzable. FISH using whole painting probes revealed that chromosomes 1 and 20 distributed on apoptotic bodies.
FISH observed localization of telomere, which controls life spans of the cells. Telomere was detected on larger apoptoticbodies. On the contrary centromeres did on smaller apoptotic bodies. Hence, morphological changes of apoptosis would start on the centromeres, which locate in the center and are characteristic each of chromosomes, and the phenomenon result into formation of apoptotic bodies.
Cultured cells from human ovarian carcinoma (KFr) and ednometrial carcinoma (Ishikawa) were exposured to CDDP and taxol, and they were harvested for analyzing of apoptotic cells. KFr showed a peak at sub-G1 fraction, and the cells in the fraction were apoptotic cells identified. Further study will follow to identify the apoptosis-related genes.
|
