1997 Fiscal Year Final Research Report Summary

Gene expression of the nitric oxide synthase in fetal organs with intrauterine growth restriction

Research Project

Project/Area Number	08671934
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Tokyo Women's Medical College (T.W.M.C)
Principal Investigator	TAKAGI Koichiro Tokyo Women's Medical College (T.W.M.C) School of Med., Associate professor, 医学部, 助教授 (90154749)
Co-Investigator(Kenkyū-buntansha)	SAKAI Keiji Tokyo Women's Medical College (T.W.M.C) School of Med., Resident, 医学部, 助手 (30260962) MURAOKA Mitsue Tokyo Women's Medical College (T.W.M.C) School of Med., Assistant professor, 医学部, 講師 (60147387)
Project Period (FY)	1996 – 1997
Keywords	nitric oxide / pregnancy / intrauterine growth restriction / hypoxia / perinatal period
Research Abstract	Gene expression of the endothelial nitric oxide synthase (eNOS) was examined by competitive reversed polymerase chain reaction (RT-PCR) assay in rat fetal organs with intrauterine growth restriction (IUGR). Both fetuses and placentas from the uterine horns with ligation to the uterine artery were significantly smaller than those of the contralateral horns of the uteri. Messenger RNA (m RNA) abundance of eNOS in the placenta with IUGR was significantly less than that of the control. Messenger RNA abundance in the fetal brain and liver was below detection limit of our assay. Unlike preproendothelin-1 m RNA,eNOS m RNA level in the kidney with IUGR showed no significant change with hypoxia.

(4 results)