1997 Fiscal Year Final Research Report Summary
Purification of the antibody against synthetic peptide from N-myc cncoprotein
| Project/Area Number |
08672053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
小児外科
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| Research Institution | University of Tokyo |
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Principal Investigator |
OBANA Kazuko Faculty of Medicine, University of Tokyo, 医学部・附属病院, 助手 (60272580)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIDA Yoshiaki Gumma Children's medical Center, Director, 院長 (80010164)
KAMII Yoshiyuki Faculty of Medicine, University of Tokyo, 医学部・附属病院, 助手 (70177567)
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| Project Period (FY) |
1996 – 1997
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| Keywords | N-Myc oncoprotein / Synthetic peptide / multiple antigen peptide method |
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| Research Abstract |
The importance of determining N-myc oncoprotein rather than genomic N-myc amplification has been emphasized in neuroblastoma. N-myc-specific peptides, HGRGPPTAGSTAQSPG (codon. 136-151) and GVAPPRPGGRQTSGGDH (codon. 223-239), were synthesized and injected into rabbits in conjugation with either hemocyanin or lysine core (multiple antigen peptide method). Synthesized peptides conjugated to the lysine core raised more potent antibodies than those conjugated to hemocyanin. IgG against GVAPPRPGGRQTSGGDH purified by affinity column showed a precipitation line identical to that of N-myc oncoprotein present in N-myc amplified neuroblastomas, and strongly stained the nuclei of neuroblastoma cells with N-myc amplification, while it. did not react with N-myc unamplified tumors and with c-myc/L-myc amplified tumors either with immunoblot analysis or with immunostaining. A polyclonal antibody specific for a synthetic peptide from the N-Myc oncoprotein was thus obtained.
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