1997 Fiscal Year Final Research Report Summary
A Study of formation and Resorption of Bone and Teeth : An Investigation Using Osteosclerotic Mice
| Project/Area Number |
08672088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
SASAKI Takahisa Showa University, School of Dentistry, Assistant Professor, 歯学部, 助教授 (50129839)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Osteosclerosis / oc / oc mice / Bone Tissues / Tooth Enamel / Dentine / Electron microscopy / X-ray Microanalysis / Immunohistochemistry |
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| Research Abstract |
This study has attempted to clarify the mechanisms of cartilage-to-bone replacement in endochondral bone formation of long bones and of tooth morphegesis in osteosclerotic (oc/oc) mice by means of light and electron microscopy. Calcification of growth plate cartilage occurred in the central area of the longitudinal septa, but not in thin transverse septa. In oc/oc mice, multinucleated osteoclastic cells lacked both ruffled borders and clear zones facing the uncalcified cartilage, but formed only clear zones facing the calcified cartilage and bone matrix. The ruffled border was never formed in these osteoclastic cells. Phagocytosis of cartilage fragments was observed in osteoclastic cells lacking membrane ruffling. In oc/oc mice, hypocalcification detected by X-ray microanalysis was found in both enamel and dentine matrices. Both ameloblasts and odontoblasts also lacked membrane ruffling facing such hypocalcified matrices.
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