1997 Fiscal Year Final Research Report Summary
Mechanisms of osteoclastic bone resorption on an extracellular product from periodontal pathogenic microorganisms
| Project/Area Number |
08672205
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | MEIKAI UNIVERSITY |
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Principal Investigator |
KURIHARA Noriyoshi Meikai University, School of Dentistry, Lecturer, 歯学部, 講師 (10186512)
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| Project Period (FY) |
1996 – 1997
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| Keywords | osteoclast / tyrosinekinase / bone resorption / extracellular prpduct from periodontal pathogenic microorganism |
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| Research Abstract |
STK is a member of the hepatocyte growth factor (HGF) receptor family. The ligand for STK,macrophage stimulating protein (MSP) is a serum protein activated by members of the coagulation cascade. The RON gene was the murine homologue of the murine STK.In this report, we examined the role of MSP-RON in the signal pathway of human osteoclasts. Using anti-RON antibody, we detected RON expressed in multinucleated osteoclast-like cells (OCL) formed in the culture of human bone marrow cells. To determine the bone resorption, we placed OCL on ceramic calcium phosphate thin films formed on quartz supports coated slide (Millenium Biologix) and measured resorption. MSP stimulated the pit formation by OCL in a dose-dependent manner. MSP (50 ng/m1) cause a fourfold increase in pit area compared to control.
Furthermore, we examined the effects of MSP and HGF on OCL formation from purified populations of hematopoietic progenitors. OCL expressing the osteoclast phenotype were identified by their cross-reactivity with the 23c6 monoclonal antibody. HGF (50 ng/ml) stimulated the differentiation of progenitors to 23c6-positive OCL,but did not enhance the bone absorption. In contrast, MSP did not affect the proliferation of osteoclast precursors but stimulated bone resorption by OCL.We conclude that MSP signal transduction pathway plays a distinct role in the bone absorption compared with HGF.
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