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1997 Fiscal Year Final Research Report Summary

Development of Practical Synthetic Method for Phosphopeptides using Two-step Deprotecting Procedures

Research Project

Project/Area Number 08672421
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical pharmacy
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

OTAKA Akira  Kyoto University Graduate School of Pharmaceutical Sciences Associate Professor, 薬学研究科, 助教授 (20201973)

Co-Investigator(Kenkyū-buntansha) TAMAMURA Hirokazu  Kyoto University, Graduate School of Pharmaceutical Sciences Assistant Professor, 薬学研究科, 講師 (80217182)
Project Period (FY) 1996 – 1997
Keywordsphosphoamino acid / phosphopeptides / peptide synthesis / final deprotection / dimethylphosphono amino acids
Research Abstract

A protocol has been developed for the synthesis of peptides containing O-phosphorylated tyrosines, serines, and/or threonines. The procedure involves incorporation of dimethyl-protected phosphorylated amino acid derivatives into peptides using Boc chemistry and subsequent removal of Me groups using a two-step deprotection method consisting of high acidic and low acidic treatments. Optimized deprotection conditions for the protected resins were established, which consist of a combination of the first-step reagent (1 M TMSOTf-thioanisole in TFA,m-cresol, EDT) and the second-step reagent (first-step reagent + DMS-TMSOTf). Furthermore, the second-step reagent can be changed to the more effective reagent system (TMSOTf-DMS-m-cresol-EDT). Theses synthetic method afforded phosphoamino acid-containing peptide in high yield without significant accompanying side reactions. For a phosphoprotein synthesis, we developed SN1-deprotecting procedure (TFMSA-TFA-m-cresol). Treatment of protcted phosphopeptide thioester resins with this system afforded protected phosphopeptide thioesters, in which side chains of Lys and phosphoamino were protected with CLZ or Me grpoups, respectively. Synthetic studies on phosphoprotein utilizing protected phosphopeptide thioesters are in progress.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Joseph J.Barchi, Jr.et.al.: "Conformational Analysis of Cyclic Hexapeptide Designed as Constrained Ligands for the SH2 Domain of the p85 Subunit of Phosphatidylinositol-3-OH Kinase" Biopolymer. 38. 191-208 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bin Ye et.al.: "Preparation of Nα-Boc-4-O-Diethylphospho-L-azatyrosine,a Reagent Potentially Useful for the Synthesis of Signal Transduction Related Peptides" Synlett. 1996. 459-460 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Otaka et.al.: "Development of Practical Synthetic methodology Using Dimethylphosphono Amino Acid for Phosphopeptides" Peptides:Chemistry,Structure and Biology. 442-443 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atsushi Kosaki et.al.: "14-3-3β protein Associates with Insulin Receptor Substrate 1 and Decreases Insulin-stimulated Phosphatidylinositol-3-Kinase Activity in 3T3L1 Adipocytes" J.Biol.Chem.273. 940-944 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira Otaka et.al.: "Development of New Efficient Deprotecting Methodologies for the Synthesis of Phosphoamino Acids-containing Peptides" Peptides 1996. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Joseph J.Barchi, Jr.et.al.: "Conformational Analysis of Cyclic Hexapeptide Designed as Constrained Ligands for the SH2 Domain of the p85 Subunit of Phosphatidylinositol-3-OH Kinase" Biopolymer. 38. 191-208 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bin Ye et.al.: "Preparation of Nalpha-Boc-4-O-Diethylphospho-L-azatyrosine, a Reagent Potentially Useful for the Synthesis of Signal Transduction Related Peptides" Synlett. 459-460 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Otaka et.al.: "Development of a Practical Synthetic Methodology Using Dimethylphosphono Amino Acid for Phosphopeptides" Peptides : Chemistry, Structure and Biology. 442-443 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsushi Kosaki et.al.: "14-3-3beta protein Associates with Insulin Receptor Substrate 1 and Decreases Insulinstimulated Phosphatidylinositol-3-Kinase Activity in 3T3L1 Adipocytes" J.Biol.Chem.273. 940-944 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Otaka et.al.: "Development of New Efficient Deprotecting Methodologies for the Synthesis of Phosphoamino Acids-containing Peptides" Peptides 1996. (in press, ). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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