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1997 Fiscal Year Final Research Report Summary

ABNORMALITIES IN T-CELL RECEPTOR LOCUS AND T-CELL LEUKEMIA

Research Project

Project/Area Number 08672596
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionToyama University, Faculty of Engineering

Principal Investigator

ISOBE Masaharu  TOYAMA UNIVERSITY,FACULTY OF ENGINEERING,ASSOCIATE PROFESSOR, 工学部, 助教授 (70211050)

Project Period (FY) 1996 – 1997
KeywordsT-cell receptor locus / T-cell leukemia / TCL1
Research Abstract

The molecular involvement of T-cell Receptor (TCR) alpha/delta locus at chromosome 14q11.2 in a number of translocations such as t (8 ; 14) (q24 ; q11), t (14 ; 14) (q11 ; q32), inv (14) (q11 ; 32), t (11 ; 14) (q13 ; q11), t (11 ; 14) (p15 ; q11), t(10 ; 14) (q23 ; q11), an t (1 ; 14) (p32 ; q11) has been described in the HTLV-I-begative T-cell malignancies. Those breakpoints specifically occur within the Jdelta, Jalpha or Dalpha region of the TCR alpha/delta locus. However, there is no report which indicates the direct involvement of TCR alpha/delta locus adult T-cell leukemia (ATL) despite the fact of frequent occurrence of translocations at 14q11.2 region. At the first step to characterize the molicular involvement of TCR alpha/delta locus in ATL cases, we isolated 13BAC and 158 cosmid clones which cover more than 1Mb of region spanning entire TCR alpha/delta locus. We found there are 53 Valpha segment and 3 Vdelta segment in the entire TCR alpha/delta locus. Furthemore We localized the deltaRec locus in between Valpha19.1 and Vdelta2.1 and these findings suggest that the rearrngement between deltaRec and pseud Jalpha locus will cause the deltetion of Vdelta2.1-Ddelta-Jdelta-Cdelta-Vdelta3.1 region during the differentation of alpha/beta positive T-cells. By using those BAC clones as probes, we found that the translocation does occur within TCR alpha/delta locus in a case of ATL patient carrying chromosome abnormalities inv(14) (q11q32) and del (14) (q11q32).

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] H.Fushimi: "18S ribosomal RNA gene sequences of three PANAX species and the corresponding ginseng drugs." Biol.Pharm.Bull.19. 1530-1532 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fushimi: "A new approach for the identification of a Chinese traditional medicine,“Chuanxing"by 18S ribosomal RNA gene sequences." Phytomedicine. 3. 387-389 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Suga: "Orientation and characteristic movement of yeast cells induced by homogeneous electric field." Jpn.J.Appl.Phys.36. 396-402 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fushimi: "Application of PCR-RFLP and MASA analyses on 18S ribosomal RNA gene sequence for the identification of three ginseng drugs." Biol.Pharm.Bull.20. 765-769 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] F.Yamagishi: "Molecular analysis of chromosome 14q32 abnormalities in esophageal and gastric carcinoma." Annals of Cancer Research and Therapy. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Sakashita: "Amplification of the TCL1 flanking region at 14q32.1 with no TCL1 gene transcription in a patient with peripheral T-cell lymphoma." Leukemia. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 磯部 正治: "ヒト第14染色体のゲノム解析と疾患関連遺伝子の探索 蛋白核酸酵素増刊号ゲノムサイエンス" 共立出版, 7 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Fushimi, K.Komatsu, M.Isove and T.Namba: "18S ribosomal RNA gene sequences of three PANAX species and the corresponding ginseng drugs." Biol.Pharm.Bull.19. 1530-1532 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fushimi, K.Komatsu, M.Isobe and T.Namba: "A new approach for the identificaation of a Chinese traditional medicine, "Chuanxiong" by 18S ribosomal RNA gene sequences." Phytomedicine. 3. 387-389 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Suga, T.Hatakeyama and M.Isobe: "Oreintation and characteristic movement of yeast cells induced by homogeneous electric field." Jpn.J.Appl.Phys.36. 396-402 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Fushimi, K.Komatsu, M.Isobe and T.Namba: "Application of PCR-RFLP and MASA analyzes on 18S ribosomal RNA gene sequence for the identification of three ginseng drugs." Biol.Pharm.Bull.20 : (7). 765-769 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] F.Yamagishi, M.Isobe, T.Bandou, Y.Ihara, T.Saito, M.Saito, T.Sakamoto, M.Fujimaki and Z.Ogita: "Molecular analysis of chromosome 14q32 abnormalities in esophageal and gastric carcinoma." Annals of Cancer Research and Therapy. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Sakashita, H.Kobayashi, N.Satake, N.Maseki, M.Sakurai, T.Izumo, M.Isobe and Y.Kaneko: "Amplification of the TCL1 flanking region at 14q32.1 with no TCL1 gene transcription in a patient with peripheral T-cell lymphoma." Leukemia. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Isobe: "Genome analysis on Human Chromosome 14 and Searching for the Disease related Genes" Protein, Nucleic Acid and Enzyme. Vol.42. 2714-2720 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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