1997 Fiscal Year Final Research Report Summary
Evaluation of platelet activation in vivo by quantitative measurement of myosin phosphorylation.
| Project/Area Number |
08672636
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Yamanashi Medical University, Faculty of Medicine |
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Principal Investigator |
OZAKI Yukio Yamanashi Medical University, Faculty of Medicine, Professor, 医学部, 教授 (30134539)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Kaneo Yamanashi Medical University, Faculty of Medicine, Assistant, 医学部, 教務職員 (20242662)
YATOMI Yutaka Yamanashi Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (60200523)
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| Project Period (FY) |
1996 – 1997
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| Keywords | myosin phosphorylation / platelet aggregation / light scattering / diabetes mellitus |
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| Research Abstract |
We recently developed a new platelet aggregometer, based on light scattering, which can sensitively measure platelet aggregates of small size. Using this instrument, we were able to detect that spontaneous aggregation occurs in patients with diabetes mellitus. Since platelet activation is associated with myosin activation, we then attempted to measure the level of myosin phosphorylation in platelets obtained from diabetes mellitus patients. For this end, we developed a quantitative measurement of myosin phosphorylation, by using an monoclonal antibody which recognizes only the phosphorylated form of myosin, but not ordinary myosin. Previous methods of measuring myosin phosphorylation employed 32P labeling, which often induced artificial activation of platelets, and thus evalution of intact platelets was not possible. Based on our new method using anti-phosphorylated myosin antibody, we were able to evaluate the level of myosin phosphorylation of intact platelets obtained from patients with diabetes mellitus. We found that the level of myosin phosphorylation is significantly increased in patients with diabetes mellitus, and that the level of spontaneous aggregation is well correlated with that of myosin phosphorylation.
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