1998 Fiscal Year Final Research Report Summary
Localization, function and clinical significance of semen-related proteins
| Project/Area Number |
08672648
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Jichi Medical School (JMS) |
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Principal Investigator |
ITOH Yoshihisa Jichi Medical School, Dept.of Medicine, Associate Prof., 医学部, 助教授 (20129026)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Protein 1 / Basic fetoprotein / cystatin C / PSA / clara cell 10kDa protein / prostate / IgG |
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| Research Abstract |
Among proteins of semen origin studies were concentrated on the function, distribution and clinical significance of protein 1, basic fetoprotein, PSA, cystatin C and IgG.
1) Protein 1 (P1)
We have prepared recombinant P1 and demonstrated initially that P1 is a potent inhibitor of phospholipase A2 and phospholipase C.Using monoclonal antibodies, localization of p1 was immunohistochemically demonstrated in Skene's gland as well as prostate. Clinical significance of P1 was further expanded to lung diseases : P1 value in serum was decreased as a result of decreased synthesis at non-ciliated cells in the lung. Furthermore, its synthesis was decreased in patients with cancer with poor prognosis. Generally the value is elevated in BALF in sarcoidosis, in contrast decreased in asthma, indicating that P1 synthesis are associated with Thi and Th2 interregulatory function.
2) Basic fetoprotein
We have initially demonstrated the presence of basic fetoprotein in semen which proved to be structurally identical with that initially purified from placenta. The protein proved to be a new urinary nonspecific inflammatory and tumour marker.
3) PSA
PSA present in urine consists only of free form. Effects of ejaculation and exercise were investigated, Reference intervals were also set. Clinical significance of urinary PSA was none.
4) Cystatin C
Cystatin C rich in semen became a good marker for serum endogenous marker for GER.Clincial significance was under investigation.
5) Other
Stability of urine IgG was investigated. Urine IgG is of both serum and urine origin, the latter of which was derived from degradation of intact IgG, Fcgamma fragment, digested by proteases in urine.
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