1997 Fiscal Year Final Research Report Summary
A study for the suppressive effect of the peptide growth factors on radiation-induced apoptosis.
| Project/Area Number |
08680576
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KATOH Osamu Research Institute for Radiation Biology and Medicine HIROSHIMA UNIVERSITY Associate professor, 原爆放射能医学研究所, 助教授 (90214361)
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| Project Period (FY) |
1996 – 1997
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| Keywords | VEGF / apoptosis / bel family / transcription factor / zinc-finger motif |
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| Research Abstract |
We have found that peptide growth factors such as VEGF suppress the apoptotic cell death of the hematopoietic cells induced by exposure to gamma-rays.
1. To elucidate the molecular mechanisns of the suppressive effects of VEGF,we examined the mRNA level and the protein level of the bel family (BCL-2, BCL-XL,MCL-1) by Northern blot and Western blot hybridization analysis.
The mRNA level and the protein level of MCL-1 were increased in the CMK86 cells, a human leukemia cell line, stimulated by VEGF.Those of BCL-2 or BCL-XL,however, did not change by VEGF.MCL-1 may be involved in the suppressive effect of VEGF on apoptotic cell death.
2. We cloned a novel Kruppel-type zinc finger gene ZK-7, induced by VEGF from a CMK86 cDNA library. The deduced ZK-7 protein has 298 amino acids, and has KRAB (Kruppel associated box) -A box at the N-terminus and seven C_2H_2-type zinc-finger domain at the C-terminus. The function of the ZK-7 gene on the signaling pathway of popsis remains to be elucidated.
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