1997 Fiscal Year Final Research Report Summary
Ultraviolet Resonance Raman Study on Protein Structure Dynamics of Cytochrome c Oxidase
| Project/Area Number |
08680730
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Okazaki National Research Institutes |
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Principal Investigator |
OGURA Takashi Okazaki National Research Institutes, Institute for Molecular Science, Research Associate, 分子科学研究所分子構造研究系, 助手 (70183770)
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| Project Period (FY) |
1996 – 1997
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| Keywords | cytochrome c oxidase / UV resonance Raman / bioenergetics / proton pump / oxygen activation / electron transfer |
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| Research Abstract |
A high performance ultraviolet resonance Raman spectrophotometer for biological applications has been constructed. A solar-blind intensified charge-coupled device was attached to a 1260 mm single spectrograph with a 200 nm-blazed, 3600 grooves/mm grating. The 488 nm output of a mode-lacked argon ion laser was frequency doubled to obtain 244nm UV light for resonance Raman excitation. This Raman spectrophotometer has been applied to cytochrome c oxidase to probe structural changes of tyrosine and tryptophan residues upon redox and ligation-state changes. Resting oxidized, fully-reduced and mixed-valence forms of the enzyme in the presence and absence of carbonmonoxide or cyanide have been measured. It became evident that a redox change of heme a and / or Cu_A induced most prominent changes on tyrosine and tryptophan residues while ligation to heme a_3 and redox change of heme a_3 and / or Cu_B less affected the protein structures. Basic data to analyze time-resolved UV resonance Raman spectra of cytochrome c oxidase have been established.
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