1998 Fiscal Year Final Research Report Summary
ENU mutagenesis and a trial to isolate mutant alleles for specific genes located in the sex-determining region of the Japanese medaka.
| Project/Area Number |
08680788
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Niigata University |
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Principal Investigator |
SAKAIZUMI Mitsuru Professor, Department of Environmental Science, Faculty of Science, Niigata University, 理学部, 教授 (40175360)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Medaka / sex chromosome / sex-determining gene / mutagenesis / ethylnitrosourea / sex reversal |
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| Research Abstract |
Ethylnitrosourea mutagenesis of spermatogonia in male medaka was performed to isolate mutant alleles in the sex-determining region. We used a congenic strain, Hd-rR.Y^<HNI>, the male of which has a genetic background derived from the Hd-rR strain, and has only the chromosomal region including the sex-determining factor (and r locus) from the HNI strain in its Y chromosome.
1. Sex-reversed XY females were mated with XY males, and YY males were obtained. A sex-linked DNA marker SL1 was used for detection of YY males.
2. YY (RR ; red) and XY (Rr ; red) males were mutagenized with 1mM ENU and mated with non-treated XX (rr ; white) females.
3. A total of 746 offspring (402 males and 344 females) were produced from XY males. All females were white and 391 males red. Eleven white males were demonstrated to be sex-reversed XX individuals by mating expriment. All 269 male off spring from the YY males were red.
4. Thus no mutants were obtained in the presentstudy. In order to get mutant alleles in the sex-determining region, it is necessary to continue mutagenesis experiment.
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[Publications] Matsuda, M., Kusama, T., Kurihara, Y., Oshiro, T., Hamaguchi, S.Sakaizumi, M.: "Isolation of a sex chromosome-specific DNA sequence in the medaka, Oryzias latipes." Genes Genet.Syst.72. 263-268 (1997)
Description
「研究成果報告書概要(欧文)」より
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