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1997 Fiscal Year Final Research Report Summary

Regulation mechanism of synaptic input to Purkinje cell and LTD

Research Project

Project/Area Number 08680876
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

KOJIMA Hiroshi  Memory and Learning, The Institute of Physical and Chemical, Research Researcher, 記憶学習機構研究チーム, 研究員 (50281671)

Co-Investigator(Kenkyū-buntansha) YANAGIHARA Dai  Memory and Learning, The Institute of Physical and Chemical, Research Researcher, 記憶学習機構研究チーム, 研究員 (90252725)
FURUYA Shigeki  Cellular Glycobiology, The Institute of Physical and Chemical, Research Research, 糖細胞情報研究チーム, 研究員 (00222274)
Project Period (FY) 1996 – 1997
KeywordsLTD / Purkinje cell / patch clamp / AMPA receptor / computer simulation / IR-DIC
Research Abstract

In order to test the hypothesis Concerning the elementary mechanisms of long term depression of cerebellar Purkinje cells, the patch clamp experiment was conducted together with a non-stationary noise analysis and a computer simulation of excitatory postsynaptic currents recorder from somata of Purkinje cells. The obtained results were (1) The contribution of change in kinetics of AMPA receptor channels to the reduction of the amplitudes of EPSCs was small. (2) No change in single channel conductance of AMPA receptor channels was observed during LTD.(3) By using computer simulation, it was concluded that a reduction in affinity of glutamate to receptors causes LTD and that open probability of receptor channels decreased during LTD.(4) Taken together above results, it was suggested that LTD could be induced as the result of the possible decrease in the number of the active receptor channels in the postsynaptic membrane at the peak of excitatory postsynaptic currents.
The present result was obtained for the first time in LTD of Purkinje cell and was unique and important suggestions for the research field of cerebellar cortex. During the course of this experiment, it was necessary to record stable signals from a cell in the deep layer of a slice preparation, so that a newly developed Infrared DIG video microscope was designed for this purpose.

  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] KOJIMA,H and IlevaL.: "Application of Variance analysis to synaptic ・・・" From Membrane to Mind. V.1. 141 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOJIMA,H., et al.: "Non-stationary Noise Analysis and its Application・・・" Proceedings of the 1997 International ・・・・. 685-688 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOJIMA,H. et al.: "Study of the Basic mochanisms of Long-term ・・・・" Proceeding of the 1997 IEICF Transaction ・・・. 1-8 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOJIMA,H. et al.: "Development of Infrared DIC Video Microscopy-." Bioimages. 6. 9-19 (1998)

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      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi et.al.: "Ethanol directly open G-protein-activated・・" Nature. basically Accepted.

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      「研究成果報告書概要(和文)」より
  • [Publications] 小島比呂志,他: "バイオイメージング技術の最先端" 先端医療技術研究所, 400 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOJIMA.H.et,al: "Slow Synaptic Responses and Modulatins" 日本生理学会(未定), (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kojima, H., Watanabe, T.and Abe, K: "Visualizing Unstained Cells in Living Preparations by Improved Infrared DIC-Video Microscopy" RIKEN Review. No.12. 59-60 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ileva, V.L.: "Application of Variance Analysis to Synaptic Current during long-term Depression Recorded from Cerebellar Purkinji Cell of Rat. From Membrane to Mind ed. by N.Elsner and H.Wassle, p141 Georg Thieme verlag Stuttgart New York" The Proceedings of the 25^<th> Gottingen Neurobiology Conference Invited speaker. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ileva, V.L.: "Characteristics of AMPA receptor channels during long-team depression of Purkinje cell of rat." Neuroscience Research. suppl., 21. S62 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ileva, V.L., Ichikawa, K.and Traynelis, S.F.: "Non-stationary Noise Analysis and its Application to Synaptic Plasticity." Proceedings of the 1997 International Symposium on Nonlinear Theory and its. Application(NOLTA'97). 685-688 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ileva, V.L., Ichikawa, K.: "Study of the basic mechanisms of Long-term depression of rat cerebellum" Proceedings of the 1997 IEICE Transaction, ME and Biocybanetics. MBE-3. 1-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ileva, L.V., Abe, K., Yoshioka, T.and Watanabe, T.: "Development of Infrared DIC Video Microscopy and its Application to Unstained Cells in Living Preparations" Bioimages 6. No.1. 9-19 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, T., Matsushima, S., Uchimura, S., and Kojima, H: "Observation of retina of SD rat with infrared differential interference contrast video microscopy." J.Kyorin Medical Soc.29. 169 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ichikawa, K., Ileva, V.L., Traynelis, S.F.and Yoshioka, T.: "Properties of AMPA receptor channels during long-term depression in rat cerebellar Purkinje cell" The Satellite Symposium of the 75^<th> Annual Meeting of the Japanese Physiological Society on Slow Synaptic Responses and Modulations. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ichikawa, K., Ileva, V.L., Yawo, H.and Yoshioka, T.: "A study of the Glutamate receptor channels of rat cerebellar Purkinje cell. (Slide session)" Soc.Neurosci.Abstr.Vol.24, Part 2. 1272 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ichikawa, K., Ileva, V.L.and Yoshioka, T.: "Characteristics of Glutamate Receptors of Rat Cerebellar Purkinje cell" Neuroscience Research suppl.S84. 074 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, H., Kojima, H., Shibata, S.and Yoshioka, T: "Calcium Response in the Brain Nuclei Related to Auditory Fear Conditioned Learning" Neuroscience Research Suppl.S229. 482 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kobayashi, T.Ikeda, K., Kojima, H., Niki, H., Yano, R.and Kumanishi, T.: "Ethanol directly opens G-protein-activeted inwardly rectifying potassium channels." Nature(basically accepted as an Article).(1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ileva, V.L., and Santamaria, F: "Non-stationary noise analysis and its application to long-term depression." Neural Computation. (Submitted). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, H., Ileva, V.L., and Santamaria, F.: "Mechanisms of glutamate receptor channels underlie long-term depression of cerebellar Purkinji cell of rat." Journal of Neuroscience. (submitted).(1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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