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1997 Fiscal Year Final Research Report Summary

Basic study for establishment of the chromosome deletion model mouse

Research Project

Project/Area Number 08680902
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionTohoku University

Principal Investigator

MIYOSHI Ichiro  Tohoku University, School of Medicine, Research Associate, 医学部, 助手 (10183972)

Co-Investigator(Kenkyū-buntansha) KASAI Noriyuki  Tohoku University, School of Medicine, Professor, 医学部, 教授 (60001947)
Project Period (FY) 1996 – 1997
KeywordsModel Mouse / Transgenic Mouse / Chromosome Deletion / Triplet (Trinucleotide) Repeat
Research Abstract

To gain insight into the association of a chromosome deletion with a fragile site localized to the p (CCG) repeat which expand with transmission and to develop an animal model of this disorder, we have generated transgenic mice carrying p (CCG) repeats in the transgene. Uninterrupted 30-, 60-, and 100-repeats p (CCG) DNA fragment were amplified by PCR using CCG and CGG trinucleotide repeat oligomers (40repeat) as both primers and template. Constructs were prepared by ligation of these synthesized p (CCG) DNA fragments into the 5'-untranslated region of LacZ high expression vector composed of cytomegalovirus enhancer, beta-actin promoter, beta-globin intron, LacZ and polyA signal sequence. Regardless of the number of repeat used, these transgenes containing the CCG repeat in transgenic mice showed on change in repeat length with transmission. Neither the chromosome deletion nor phenotypic effects of the transgenes were observed in transgenic mice. Therefore, we may need to consider the use of longer CCG repeat in the constructs or factors other than trinucleotide repeat length alone to explain CCG instability and create a model mouse.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Nakayama K.: "Visualization of yellowish-orange Iuminescence from cuprous metallothioneins in liver of Long-Evans Cinnamon rat." Biochimica et Biophysica Acta. 1289・1. 150-158 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyoshe, I.: "A solid phase enzyme-linked assay for ceramide glycanase using GMI and novel β-galactosidase inhibitor." Analytical Biochemistry. 236. 360-363 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kon, Y: "Morphological study of pituitary tumorigenesis in transgenic mice induced by hybrid oncogene of thyrotropin β-subinit and the simian virus 40 large T antigen." Histology and Histopathology. 12. 981-990 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita, T.: "Progressive effect of α-phenyl-N-tert-butyl nitrone(PBN) on rat embryo development in vitro." Free Radical Biology & Medicine. 23. 1073-1077 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shikishima, H: "HTLV-I pX transgenic rtas:development of cytokine-producing mammarycarcinomas and establishment of the pX mammary carcinoma cell lines." Leukemia. 11・Suppl.3. 70-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamazaki, H: "A wide spectrum of collagen vascular and autoimmune diseases in transgenic rats carrying the env-pX gene of human T lymphocyte virus typeI." International Immunology. 9. 339-346 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama, K.: "Visualization of yellowish-orange luminescence from cuprous metallothioneins in liver of Long-Evans Cinnamon rat." Biochimica et Biophysica Acta. 1289 (1). 150-158 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyoshi, I.: "A solid phase enzyme-linked assay for ceramide glycanase using GM1 and a novel beta-galactosidase inhibitor." Analytical Biochemistry. 236. 360-363 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita, T.: "The effects of alpha-phenyl-tetra-butyl nitrone (PBN) on copper-induced rat fulminant hepatitis with jaundice." Free Radical Biology & Medicine. 21 (6). 755-761 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kon, Y.: "Morphological study of pituitary tumorigenesis in transgenic mice induced by hybrid oncogene of thyrotropin beta-subunit and the simian virus 40 large T antigen." Histology and Histopathology. 12. 981-990 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita, T.: "Progressive effect of alpha-phenyl-N-tert-butyl nitrone (PBN) on rat embryo development in vitro." Free Radical Biology & Medicine. 23. 1073-1077 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shikishima, H: "HTVL-I pX transgenic rats : development of cytokine-producing mammarydinomas and establishment of the pX mammary carcinoma cell lines." Leukemia. 11 Suppl 3. 70-72 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamazaki, H: "A wide spectrum of collagen vascular and autoimmune diseases in transgenic rats carrying the env-pX gene of human T lymphocyte virus type I." International Immunology. 9. 339-346 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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