1996 Fiscal Year Annual Research Report

魚類におけるメラトニン受容体の分布と機能

Research Project

Project/Area Number	08760190
Research Institution	St. Marianna University School of Medicine
Principal Investigator	飯郷雅之聖マリアンナ医科大学, 医学部, 助手 (10232109)
Keywords	メラトニン / メラトニン受容体 / サーカディアンリズム
Research Abstract	魚類の光受容器官である松果体と網膜において合成されるホルモン,メラトニンは環境の明暗と生物時計の制御を受けて合成され,様々な生体リズムの制御に関与していると考えられている.本研究においては,魚類におけるメラトニンの生理機能を解明するための基礎実験として,メラトニン受容体の分布と機能について解析することを試みた. 1.メラトニン受容体の分布オートラジオグラフィーによる解析.2-[^<125>I]iodomelatoninをリガンドとしてin vitroオートラジオグラフィーによりキンギョ脳内メラトニン受容体の局在を解析した結果,メラトニン受容体は視蓋,小脳分子層,視床下部,網膜に高密度に存在することが明らかになった. 2.メラトニン受容体遺伝子のクローニング.これまでに報告された脊椎動物のメラトニン受容体遺伝子の塩基配列をもとにdegenerate primerを作成し,キンギョ脳total RNAを鋳型にPCR法によりcDNAクローニングを行った.その結果,4種のメラトニン受容体をコードすると思われる塩基配列を増幅することができた.これらのクローンは脊椎動物のメラトニン受容体の3番目の膜貫通部位の3′-側から7番目の膜貫通部位に相当するもので,2つはMel_<1a>サブタイプを,他の2つはMel_<1b>サブタイプをコードしていることがhomology searchの結果より明らかになった.全長をコードする遺伝子のクローニングを現在行っている.

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] Aduchez-Vazquez et al.: "Demand-feeding and loudmotor circadiau Hythms in the goldlish,auassius aura tus : Dual and independent feeding." Physiology and Behavior. 60. 665-674 (1996)
[Publications] Iigo and Tabata: "Circadian Hythms of locomotor activity in the goldfish,carassius aura tus." Physiology and Behavior. 60. 775-781 (1996)
[Publications] Suzuki et al.: "Seasoual chauges in blood IgM leucls in goldfish,with special reference to water temperature and gonadal maturation." Fisheries Science. 62. 754-759 (1996)
[Publications] Ohtani Kaneko et al.: "Nerve growth factor (NGF) induces increuse in multiubiqnitin chains and concomitant decrease in tree ubiquitin in nuclei of PC12h." Neuroscience Research. 26. 349-355 (1996)
[Publications] Iigo and Tabata: "Circadian Hythms of locomotor activity in the rainbow trout,oucornyuchus mykiss." Fisheries Sucince. 63. 77-80 (1997)
[Publications] Iigo et al.: "Regulation of guanine nucleotiaes and cations of melutouin binding sites in the goldfish brain." Biological Signals. (in press).
[Publications] Iigo et al.: "Ocular melatonin rhythms in the goldfish,Carassius anratus." Journal of Biological Rhythms. (in press).
[Publications] Iigo et al.: "Regulation by light and darkwess of melatonin secretion from the superfused masu Salmon (onerthuchus masou) pineal organ." Biological Rhythm Research. (in press).
[Publications] Iigo et al.: "Dcular melatonin rhythms in a cypriuid teleost,oikawa Zacuo platypus,are drivln by light-dark cycles," Zoological Science. (in press).
[Publications] Sauchez-Vazquez et al.: "Daily cycles in plasma and ocular melatonin in Demaud-fed sea bass,Dicen trarchus labrax,L." Journal of Comparative Physiology B. (in press).
[Publications] Iigo et al.: "Unusual responses to light and darkness of ocular melatonin in European sea bass" Neuroreport. (in press).
[Publications] Chiba et al.: "Involvement of the pineal glaud in acute immobilization-related suppession of naloxone induced LH release in ovariec tomized estrogan-prined rats." Journal of Neuroendocrinology. (in press).

1996 Fiscal Year Annual Research Report

魚類におけるメラトニン受容体の分布と機能

Principal Investigator

飯郷 雅之 聖マリアンナ医科大学, 医学部, 助手 (10232109)

Research Products

[Publications] Aduchez-Vazquez et al.: "Demand-feeding and loudmotor circadiau Hythms in the goldlish,auassius aura tus : Dual and independent feeding." Physiology and Behavior. 60. 665-674 (1996)

[Publications] Iigo and Tabata: "Circadian Hythms of locomotor activity in the goldfish,carassius aura tus." Physiology and Behavior. 60. 775-781 (1996)

[Publications] Suzuki et al.: "Seasoual chauges in blood IgM leucls in goldfish,with special reference to water temperature and gonadal maturation." Fisheries Science. 62. 754-759 (1996)

[Publications] Ohtani Kaneko et al.: "Nerve growth factor (NGF) induces increuse in multiubiqnitin chains and concomitant decrease in tree ubiquitin in nuclei of PC12h." Neuroscience Research. 26. 349-355 (1996)

[Publications] Iigo and Tabata: "Circadian Hythms of locomotor activity in the rainbow trout,oucornyuchus mykiss." Fisheries Sucince. 63. 77-80 (1997)

[Publications] Iigo et al.: "Regulation of guanine nucleotiaes and cations of melutouin binding sites in the goldfish brain." Biological Signals. (in press).

[Publications] Iigo et al.: "Ocular melatonin rhythms in the goldfish,Carassius anratus." Journal of Biological Rhythms. (in press).

[Publications] Iigo et al.: "Regulation by light and darkwess of melatonin secretion from the superfused masu Salmon (onerthuchus masou) pineal organ." Biological Rhythm Research. (in press).

[Publications] Iigo et al.: "Dcular melatonin rhythms in a cypriuid teleost,oikawa Zacuo platypus,are drivln by light-dark cycles," Zoological Science. (in press).

[Publications] Sauchez-Vazquez et al.: "Daily cycles in plasma and ocular melatonin in Demaud-fed sea bass,Dicen trarchus labrax,L." Journal of Comparative Physiology B. (in press).

[Publications] Iigo et al.: "Unusual responses to light and darkness of ocular melatonin in European sea bass" Neuroreport. (in press).

[Publications] Chiba et al.: "Involvement of the pineal glaud in acute immobilization-related suppession of naloxone induced LH release in ovariec tomized estrogan-prined rats." Journal of Neuroendocrinology. (in press).

飯郷雅之聖マリアンナ医科大学, 医学部, 助手 (10232109)