1996 Fiscal Year Annual Research Report

重金属の鼻粘膜細胞のシグナル伝達に与える影響

Research Project

Project/Area Number	08771399
Research Institution	Nagoya University
Principal Investigator	加藤昌志名古屋大学, 医学部, 助手 (10281073)
Keywords	スギ花粉症 / 可溶性接着分子 / サイトカイン / 塩化第二水銀 / 細胞内シグナル伝達 / チロシンリン酸化蛋白
Research Abstract	1.スギ花粉症患者について花粉の飛散する約1ヵ月前(1月)、飛散前期(2月)、飛散中期(3月)、飛散後期(4月)および飛散終了後約1ヵ月(5月)の5回、血清および鼻腔洗浄液を採取し、これらの含まれている可溶性接着分子(可溶性ICAM-1)、可溶性VCAM-1、可溶性E-selectin、可溶性L-selectin)、およびIL-4を市販のELISAキットを用いて測定した。血清中の可溶性ICAM-1および可溶性VCM-1は飛散前期に有意に上昇し、可溶性L-selectinは逆に同時期に有意に低下した[Kato M,et al.Arch Otolaryngol (in press)]。一方、可溶性E-selectinは全期間を通してほとんど変化しなかった。鼻腔洗浄液中の可溶性ICAM-1は、飛散中期に最高値に達した[Kato M,et al.Allergy 51:128-132,1996]。鼻腔洗浄中の可溶性VCAM-1、可溶性E-selectin、可溶性L-selectinは測定下限値以下であった。さらに、血清および鼻腔洗浄液中のIL-4は飛散中期から飛散終了後約1ヵ月にかけて上昇し、飛散中期および飛散後期において血清中の可溶性ICAM-1と負の相関関係を示した[Kato M,et al.Ann Oto Rhino Laryn(in press)]。以上より、可溶性接着分子およびIL-4は自然飛散状態でのスギ花粉症の病態に深く関与している可能性が示唆された。 2.大気汚染物質の一つである重金属(塩化第二水銀)が細胞の活性化にどのような影響を与えるかについて、特に細胞内シグナル伝達の初期段階である蛋白のチリシンリン酸化にターゲットをしぼって解析した。塩化第二水銀はin vivo[Pu M,Akand AA,Kato M,et al.J Cell Biochem 63:104-114,1996]およびin vitro[Pu M,Akhand A Anwarul,Kato M,et al.Oncogene (in press)]でSrc高発現マウス線維芽細胞のc-Srcの自己リン酸化を著しく促進した。これは塩化第二水銀が細胞を著しく活性化する可能性を示唆している。

Research Products
(8 results)

All Other

All Publications (8 results)

[Publications] Kato M,Hattori T,Ikeda R,et al: "Amount of pollen has an effect on the systemic and local levels of soluble ICAM-1 in patients with seasonal allergic rhinitis" Allergy. 51. 128-132 (1996)
[Publications] Pu M,Akhand AA,Kato M,et al.: "Mercuric chloride mediates a protein sulfhydryl modification-based pathway of signal transduction for activating Src Kinase" J Cell Biochem. 63. 104-114 (1996)
[Publications] Kato M,Hattori T,Mastumoto Y,et al.: "Dynamics of soluble adhesion molecules level in the patients with pollinosis" Arch Otolaryngol. (in press).
[Publications] Pu M,Akhand AA,Kato M,et al: "Evidence of novel redox-linked activation mechanism for the Src Kinase with in independent of tyrosine 527-mediated segulation" oncogene. (in press).
[Publications] Kato M,Hattori T,Yamashita T,etal.: "Evidence of-potential regulation by interleukin-4 of sduble intercellular adhesion molecule-1 level in patients with seasonal allogric rhinitis" Ann Oto Rhinol Lancyn. (in press).
[Publications] Kato M,Hattori T,Kitamura M,et al.: "ICAM-1 sduvel comoum regulador da reacao alergica nasal sof estimulacao alergenica natural" Digest Alergia. 4. 12-13 (1996)
[Publications] Nkashima I,Ichihara M,Daiki,Isobe K,Kato M,et al: "Proceeclings of the Jakarta International Cancer Conference" Transgenic mice model for anti-tumor immunity studies.(in press),
[Publications] Nakashima I,Pu M,Hamaguchi M,Akhand AA,Kato M.: "Proceedings of the Jakarta International Conference" Sulfhydryl-based regulation of Src family protein tyrosine Kinases for cell growth and death (in press),

1996 Fiscal Year Annual Research Report

重金属の鼻粘膜細胞のシグナル伝達に与える影響

Principal Investigator

加藤 昌志 名古屋大学, 医学部, 助手 (10281073)

Research Products

[Publications] Kato M,Hattori T,Ikeda R,et al: "Amount of pollen has an effect on the systemic and local levels of soluble ICAM-1 in patients with seasonal allergic rhinitis" Allergy. 51. 128-132 (1996)

[Publications] Pu M,Akhand AA,Kato M,et al.: "Mercuric chloride mediates a protein sulfhydryl modification-based pathway of signal transduction for activating Src Kinase" J Cell Biochem. 63. 104-114 (1996)

[Publications] Kato M,Hattori T,Mastumoto Y,et al.: "Dynamics of soluble adhesion molecules level in the patients with pollinosis" Arch Otolaryngol. (in press).

[Publications] Pu M,Akhand AA,Kato M,et al: "Evidence of novel redox-linked activation mechanism for the Src Kinase with in independent of tyrosine 527-mediated segulation" oncogene. (in press).

[Publications] Kato M,Hattori T,Yamashita T,etal.: "Evidence of-potential regulation by interleukin-4 of sduble intercellular adhesion molecule-1 level in patients with seasonal allogric rhinitis" Ann Oto Rhinol Lancyn. (in press).

[Publications] Kato M,Hattori T,Kitamura M,et al.: "ICAM-1 sduvel comoum regulador da reacao alergica nasal sof estimulacao alergenica natural" Digest Alergia. 4. 12-13 (1996)

[Publications] Nkashima I,Ichihara M,Daiki,Isobe K,Kato M,et al: "Proceeclings of the Jakarta International Cancer Conference" Transgenic mice model for anti-tumor immunity studies.(in press),

[Publications] Nakashima I,Pu M,Hamaguchi M,Akhand AA,Kato M.: "Proceedings of the Jakarta International Conference" Sulfhydryl-based regulation of Src family protein tyrosine Kinases for cell growth and death (in press),

加藤昌志名古屋大学, 医学部, 助手 (10281073)