1997 Fiscal Year Final Research Report Summary
Molecular mechanism of daikadaptation regulated by rhodopsin phosphorylation
| Project/Area Number |
08836009
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
光生物学
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
OHGURO Hiroshi Sapporo Medical University, Department of Ophthalmology, assistant professor, 医学部, 講師 (30203748)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Takashi Sapporo Medical University, Department of Ophthalmology, professor, 医学部, 教授 (60045367)
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| Project Period (FY) |
1996 – 1997
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| Keywords | rhodopsin / G-protein cerydod reaph / rhodopsin kinase / phosphorylation / retiuitis pigucutosa / adopfation / dephosphorylation |
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| Research Abstract |
To understnad the relationship between rhodopsin phosphorylation and dark adaptation, kinetics of rhodopsin phosphorylation was investigated under several conditions and obtained following results.
1)In vivo, rhodopsin phosphorylation was occurred at two different sites and the kinetics of phosphorylation and dephosphorylation was different with each others.
2)Rhodopsin phosphorylation was catalyzed only rhodopsin kinase not protein kinase C.
3)The distribution and kinetics of phosphorylation was modified by the truncation of the C-terminus of rhodopsin.
4)The dephosphorylation of the phospho Ser located C-terminus was faster than those located near membrane.
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