1998 Fiscal Year Annual Research Report

アメリカ大陸を中心としたがん研究先進グループとの研究交流

Research Project

Project/Area Number	09042002
Research Category	Grant-in-Aid for international Scientific Research
Research Institution	The University of Tokyo
Principal Investigator	山本雅東京大学, 医科学研究所, 教授 (40134621)
Co-Investigator(Kenkyū-buntansha)	本間美和子福島県立医科大学, 医学部, 助手 (40192538) 細川好孝愛知県がんセンター, 化学療法部, 室長 (60229193) 白澤専二九州大学, 生体防御医学研究所, 助手 (10253535) 菊池章広島大学, 医学部, 教授 (10204827) 生田和良北海道大学, 免疫科学研究所, 教授 (60127181)
Keywords	国際学術 / 海外派遣 / ウイルス発癌 / シグナル伝達 / 構造生物学 / ゲノム医学 / 癌治療 / 化学発癌
Research Abstract	近年の癌の基礎研究の進展は、癌発症の機構についての理解を深める上で大きく貢献していると共に、癌の診断・治療に関して新しい方法の開発.導入可能にしている。この様な進歩に対する我が国の研究者の貢献は大きい。しかしながら、発癌に関わると思われる遺伝子で未同定のものが多く残されていることも事実であり、近代的なゲノム解析の手法を利用しながら癌関連遺伝子の探索が進められている。また米国で先駆的に進められているDNAチップを用いた解析により、発癌に関する理解が深められてきている。地理的に孤立している我が国の研究者は、自らの研究成果を欧米の研究者と討議しまた欧米の研究成果を速やかに収集して、国際的な視野で研究を推進する必要がある。そのために本研究では、我が国の研究者をアメリカ、カナダの先進的がん研究の場に送り込み、個々の研究者から又研究集会から積極的な情報収集を図った。特に、基礎研究の情報交換、シグナル伝達分子の立体構造解析に基づく癌治療薬の開発に関する情報収集と遺伝子治療等の治療法に関する情報交換、モノクローナル抗体を用いたがん治療やヒトゲノムプロジェクトの中に位置するがん研究の現状に関する情報交換に重点を置いた。非派遣者は、「がん特定研究」総括班の意見を参考にして選んだ。そして、基礎研究から臨床研究まで幅広い領域の若手研究者を中心に、22名の研究者をアメリカ合衆国に派遣し、癌の分子生物学的基礎研究、新しい治療法に関する研究、ゲノム医学に関する交流を行って、癌研究の先端的推進に貢献した。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] Y.Yoshida: "ANA,anovel member of Tob/BTG1 family,is expressed in the ventricular zone of the developing central nervous system" Oncogene. 16. 2687-2693 (1998)
[Publications] C.Kimura: "Mechanism of UV-induced apoptosis in human Leukemia cells:roles of Ca^<2+>/Mg^<2+>-dependnet endonuclease,caspase-3,and stress-activated protein kinases" Experimental Cell Research. 239. 411-422 (1998)
[Publications] M.Ohba: "Induction of differentiation in normal human keratinocytes by adenovirus-mediated introduction of the μ and γ isoforms of protein kinase C" Molecular ad Cellular Biology. Sept.5199-5207 (1998)
[Publications] T.Sasaki: "ATM mutatiohns in patients with ataxia telangiectasia screened by a hietarchial strategy" Human Mutation. 12. 186-195 (1998)
[Publications] N.Senmaru: "Suppression of Erk activation and in vivo growth in esophageal cancer cells by the dominant negative Ras mutant,N116Y" Intl.J.Cancer. 78. 366-371 (1998)
[Publications] M.Masuda: "Effects of procollagen C-proteinase enhancer protein on the growth of cultured rat fibroblasts revealed by an excisable retroviral vector" Cell Growth & Differentiation. 9. 381-391 (1998)
[Publications] F.Okada: "Impact of oncogenes in tumor angiogenesis:Mutant K-ras up-regulation of vascular endothelial growth factor/vascular permeability factor is necessary,but not sufficient for tumorigenicity of human colorectal carcinoma cells" Proc.Natl.Acad.Sci.USA. 95. 3609-3614 (1998)
[Publications] M.Tobiumi: "Dependence on host cell cycle for activation of human immunodeficiency virus type 1 gene expression from latency" J.of General Virol.79. 1363-1371 (1998)
[Publications] M.Yamashita: "Requirement for p56^<lck> tyrosine kinase activation in T_h subset differentiation" International Immunology. 10. 577-591 (1998)
[Publications] D.Fan: "Boral recovery in 6-hydroxydopamine-lesioned rats by cotransduction of striatum iwth tyrosine hydroxylase and aromatic L-amino acid decarboxylase genes using two separate adeno^associated fvirus vectors" Human Gene Therapy. 9. 2527-2535 (1998)
[Publications] N.Suwabe: "GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitoro ES cell differentiation" Blood. 92. 4108-4118 (1998)
[Publications] T.Mitaka: "The current status of primary hepatocyte culture" Intl.J.Exp.Path.79. 393-409 (1998)
[Publications] T.Kanno: "Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/core binding factor α subunit revealed in the presence of the β subunite" Molecular nd Cellular Biology. 18. 2444-2454 (1998)
[Publications] H.Inoue: "Interplay of signal mediators of decapentaplegic(Dpp):molecular characterization of mothers against dpp,medea,and daughters against dpp" Mol.Biol.Cell. 9. 2145-2156 (1998)
[Publications] S.Ikeda: "Axin,a negative regulator of the Wnt signaling pathway,forms a complex with GSK-3β and β-catenin and promotes GSK-3β-dependent phosphorylation of β-catenin" EMBO J.17. 1371-1384 (1998)
[Publications] Y.Yoshida: "Decreased dcc mRNA expression in human gastric cancers is clinicopathologically significant" Intl.J.Cancer. 79. 634-639 (1998)
[Publications] M.Nonaka: "Opsonic complement component C3 in the solitary ascidian,halocynthia roretzi" J.of Immunol.162. 387-391 (1999)
[Publications] N.Tsukamoto: "Two differently regulated nuclear factor κB activation pathways triggered by the cytoplasmic tail of CD40" Proc.Natl.Acad.Sci.USA. 96. 1234-1239 (1999)
[Publications] T.Joh: "Establishment of an inducible expression system of chimeric MLL-LTG9 protein and inhibition of Hox a7,Hox b7 and Hox c9 expression by MLL-LTG9 in 32Dc13 cells" Oncogene. (in press). (1999)

1998 Fiscal Year Annual Research Report

アメリカ大陸を中心としたがん研究先進グループとの研究交流

Principal Investigator

山本 雅 東京大学, 医科学研究所, 教授 (40134621)

Research Products

[Publications] Y.Yoshida: "ANA,anovel member of Tob/BTG1 family,is expressed in the ventricular zone of the developing central nervous system" Oncogene. 16. 2687-2693 (1998)

[Publications] C.Kimura: "Mechanism of UV-induced apoptosis in human Leukemia cells:roles of Ca^<2+>/Mg^<2+>-dependnet endonuclease,caspase-3,and stress-activated protein kinases" Experimental Cell Research. 239. 411-422 (1998)

[Publications] M.Ohba: "Induction of differentiation in normal human keratinocytes by adenovirus-mediated introduction of the μ and γ isoforms of protein kinase C" Molecular ad Cellular Biology. Sept.5199-5207 (1998)

[Publications] T.Sasaki: "ATM mutatiohns in patients with ataxia telangiectasia screened by a hietarchial strategy" Human Mutation. 12. 186-195 (1998)

[Publications] N.Senmaru: "Suppression of Erk activation and in vivo growth in esophageal cancer cells by the dominant negative Ras mutant,N116Y" Intl.J.Cancer. 78. 366-371 (1998)

[Publications] M.Masuda: "Effects of procollagen C-proteinase enhancer protein on the growth of cultured rat fibroblasts revealed by an excisable retroviral vector" Cell Growth & Differentiation. 9. 381-391 (1998)

[Publications] F.Okada: "Impact of oncogenes in tumor angiogenesis:Mutant K-ras up-regulation of vascular endothelial growth factor/vascular permeability factor is necessary,but not sufficient for tumorigenicity of human colorectal carcinoma cells" Proc.Natl.Acad.Sci.USA. 95. 3609-3614 (1998)

[Publications] M.Tobiumi: "Dependence on host cell cycle for activation of human immunodeficiency virus type 1 gene expression from latency" J.of General Virol.79. 1363-1371 (1998)

[Publications] M.Yamashita: "Requirement for p56^<lck> tyrosine kinase activation in T_h subset differentiation" International Immunology. 10. 577-591 (1998)

[Publications] D.Fan: "Boral recovery in 6-hydroxydopamine-lesioned rats by cotransduction of striatum iwth tyrosine hydroxylase and aromatic L-amino acid decarboxylase genes using two separate adeno^associated fvirus vectors" Human Gene Therapy. 9. 2527-2535 (1998)

[Publications] N.Suwabe: "GATA-1 regulates growth and differentiation of definitive erythroid lineage cells during in vitoro ES cell differentiation" Blood. 92. 4108-4118 (1998)

[Publications] T.Mitaka: "The current status of primary hepatocyte culture" Intl.J.Exp.Path.79. 393-409 (1998)

[Publications] T.Kanno: "Intrinsic transcriptional activation-inhibition domains of the polyomavirus enhancer binding protein 2/core binding factor α subunit revealed in the presence of the β subunite" Molecular nd Cellular Biology. 18. 2444-2454 (1998)

[Publications] H.Inoue: "Interplay of signal mediators of decapentaplegic(Dpp):molecular characterization of mothers against dpp,medea,and daughters against dpp" Mol.Biol.Cell. 9. 2145-2156 (1998)

[Publications] S.Ikeda: "Axin,a negative regulator of the Wnt signaling pathway,forms a complex with GSK-3β and β-catenin and promotes GSK-3β-dependent phosphorylation of β-catenin" EMBO J.17. 1371-1384 (1998)

[Publications] Y.Yoshida: "Decreased dcc mRNA expression in human gastric cancers is clinicopathologically significant" Intl.J.Cancer. 79. 634-639 (1998)

[Publications] M.Nonaka: "Opsonic complement component C3 in the solitary ascidian,halocynthia roretzi" J.of Immunol.162. 387-391 (1999)

[Publications] N.Tsukamoto: "Two differently regulated nuclear factor κB activation pathways triggered by the cytoplasmic tail of CD40" Proc.Natl.Acad.Sci.USA. 96. 1234-1239 (1999)

[Publications] T.Joh: "Establishment of an inducible expression system of chimeric MLL-LTG9 protein and inhibition of Hox a7,Hox b7 and Hox c9 expression by MLL-LTG9 in 32Dc13 cells" Oncogene. (in press). (1999)

山本雅東京大学, 医科学研究所, 教授 (40134621)