1997 Fiscal Year Final Research Report Summary
The Search for Natural Anticancer Substances in East Africa
| Project/Area Number |
09042012
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Special Cancer Research |
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| Research Field |
Chemical pharmacy
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
KATO Atsusi Kobe Pharmaceutical University, Pharmacy, Professor, 薬学部, 教授 (50068326)
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| Co-Investigator(Kenkyū-buntansha) |
JUMA F. D. Nairobi University, Medicine, Professor, 医学部, 教授
NISHIYAMA Yumi Kobe Pharmaceutical University, Pharmacy, Resaerch Assistant, 薬学部, 助手 (30248116)
TOKUDA Harukuni Kobe Prefectural University of Medicine, Medicine, Lecturer, 医学部, 助手 (60111960)
アルバート R. チンバザサ動植物園, 園長, 助教授
MATHENGE S. G. Nairobi University, Medicine, Professor, 理学部, 講師
ALBERT R Tsimbazaza Zoological and Botanical Garden, Director, Associate Professor
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| Project Period (FY) |
1997
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| Keywords | Hypoxis angustifolia / Hypoxis acrea / traditional medicines / anticancer promotion activity / HCC-panel / Uvaria spp. / dihydrochalcon / anticancer activity |
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| Research Abstract |
1. The research of natural medicinal resources for search of anticancer substances in Madagascar : The research areas with traditional herbal medicines and with unused plants were limited to Fort-Dauphin, Ambosary and Morondava district in Madagascar. The 40 plant species were collected in these areas during out field works. We tried to screening by anticancer promoting activity for 3 species selected from among the collection plants. From the results of this test on each methanol extract of the 3 plants, two kind plants, Hypoxis angustifolia (root) and RESONDRO (vernacular name) showed anticancer promotion activity and these data were as followed ; the former control % and (viability %) were 30.8 (70) on 10μ/ml (conc., ) and 70.2 (70) on 1μ/ml. The latter was 30.2 (60) on 10μ/ml and 80.4 (60) on 1μ/ml. The test were carried out under the condition of TPA (20 ng/ml)=100%.
2. Anticancer activation test of the chalcon compounds isolated from Uvaria acuminata (Annonaceae) : We detemined th
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e structures of six new compounds and six known compounds from Uvaria acuminata, which were dihydrochalcons and acetogenins. Giliama people of southeast region in Kenya use this plant for traditional medicine of painkiller or antibacterial agent. Two dihydrochalcons, 5''''-(2''''-hydroxybenzyl)-diuvaretin and 5'''-(2''''-hydroxybenzyl)-diuvaretin were afforded for anticancer screening test using HCC-Panel (human cell line panel). Then, the data computerized from the HCC-panel screening system showed a unique Mean Graph (Finger Prints), and it was suggested that the compound possess a unique anticancer active mechanism. On the other hand, these anticancer active dihydroxybenzly chalcons in HCC-panel system inhibited Topo-I and -II isomelase action. The comparison between the HCC-panel activity and Topoisomelase inhibition of a same compound is of interest from the viewpoint of correlation between chemical structure and bioactive mechanism of these compounds. Further, we isolated a new pyrene compound from Uvaria lucida of another species of genus Uvaria. The structure of this compound was particularly elucidated by spectroscopic analysis. The compound appeared inhibitory effect to cancer promotion on Epstein-Barr Virus (EBV). The inhibitory effect the pyrene compound on EBV activation ; control % (viability %) =50.9 (60) on 10μg/ml. Less
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