1999 Fiscal Year Final Research Report Summary
EVOLUTIONARY AND CELLH BIOLOGICAL STUDIES ON Wolbachia, ENDOCELLULAR SYMBIOTIC MICROORGANISMS
| Project/Area Number |
09044203
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
動物生理・代謝
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
ISHIKAWA Hajime GRADUAGTE SCHOOL OF SCIENCE, THE UNIVERSITY OF TOKYO, PROFESSOR, 大学院・理学系研究科, 教授 (70012482)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHIZAKI Sugihiko GRADUAGTE SCHOOL OF AGRICULTURE, THE UNIVERSITY OF TOKYO, INSTRUCTOR, 大学院・農学生命科学研究科, 助手 (10270894)
MORIOKA Mizue GRADUAGTE SCHOOL OF SCIENCE, THE UNIVERSITY OF TOKYO, INSTRUCTOR, 大学院・理学系研究科, 助手 (20272461)
SASAKI Tetsuko GRADUAGTE SCHOOL OF SCIENCE, THE UNIVERSITY OF TOKYO, INSTRUCTOR, 大学院・理学系研究科, 助手 (60235257)
MIURA Kazuki CHUGOKU EXPERIMENTAL SATION OF AGRICULTURE CHIEFINVESTIGATOR, 中国農業試験場, 主任研究官
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| Project Period (FY) |
1997 – 1999
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| Keywords | CYTOPLASMIC INCOMPATIBILITY / INDIAN MEAL MOTH / ALMOND MOTH / MEDITERRANEAN FLOUR MOTH / PHYLOGENETIC POSITION / WSP GENE / INCOMPATIBLE CROSS / CELL SURFACE PROTEIN |
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| Research Abstract |
Wolbachia are a group of inherited Rickettia-like bacteria found in a number of arthropods and known to cause various reproductive alterations of their hosts, including feminization, parthenogenesis and cytoplasmic incompatibility. We examined Wolbachia infection in three species of moths belonging to the sub-family Phycitinae, the Indian meal moth Plodia interpunctella, the almond moth Ephestia cautella and the Mediterranean flour moth Ephestia kuehniella. We detected infections in E. cautella and two strains of E. kuehniella, one from Tsuchiura city and the other from Yokohama city. Wolbachia was not detected in P. interpunctella. The phylogenetic positions of Wolbachia harbored by E. cautella and E. kuehniella were estimated based on the sequences of the wsp gene that encodes a Wolbachia cell surface protein. We also performed crossing experiments to examine cytoplasmic incompatibility. It was shown that Wolbachia in E. cautella caused complete cytoplasmic incompatibility : no egg-hatch was observed in the cross between infected males and uninfected females. Both Tsuchiura and Yokohama strains of E. kuehniella showed partial cytoplasmic incompatibility, but the levels were significantly different between the two strains. The rates of egg hatch in the incompatible crosses within Tsuchiura and Yokohama strains were 60.8% and 16.9%, respectively.
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